BMRB Entry 18829

Title:
NMR restraints for the C-terminal RRM domain (RRM3) of TIA-1 protein.
Deposition date:
2012-11-07
Original release date:
2013-08-14
Authors:
Cruz-Gallardo, Isabel; Aroca, Angeles; Persson, Cecilia; Karlsson, Goran; Diaz-Moreno, Irene
Citation:

Citation: Cruz-Gallardo, Isabel; Aroca, Angeles; Persson, Cecilia; Karlsson, B. Goran; Diaz-Moreno, Irene. "RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions."  J. Biol. Chem. 288, 25986-25994 (2013).
PubMed: 23902765

Assembly members:

Assembly members:
RRM_domain, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-11

Data sets:
Data typeCount
13C chemical shifts199
15N chemical shifts97
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C terminal RRM domain (RRM3)1

Entities:

Entity 1, C terminal RRM domain (RRM3) 103 residues - Formula weight is not available

1   SERGLYSERSERTYRASPGLUVALVALASN
2   GLNSERSERPROSERASNCYSTHRVALTYR
3   CYSGLYGLYVALTHRSERGLYLEUTHRGLU
4   GLNLEUMETARGGLNTHRPHESERPROPHE
5   GLYGLNILEMETGLUILEARGVALPHEPRO
6   ASPLYSGLYTYRSERPHEVALARGPHEASN
7   SERHISGLUSERALAALAHISALAILEVAL
8   SERVALASNGLYTHRTHRILEGLUGLYHIS
9   VALVALLYSCYSTYRTRPGLYLYSGLUTHR
10   LEUASPMETILEASNPROVALGLNGLNGLN
11   ASNGLNILE

Samples:

sample_1: RRM domain, [U-13C; U-15N], 0.6 mM; potassium phosphate 20 mM; potassium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

GB EDK99168 EPY74867
REF XP_003481239 XP_006505956 XP_009182642 XP_011239588 XP_011239589

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks