BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18822

Title: Solution Structure of the trans-membrane domain of the NS2A of dengue virus   PubMed: 23408612

Authors: Gayen, Shovanlal; Kang, Congbao

Citation: Xie, Xuping; Gayen, Shovanlal; Kang, Congbao; Yuan, Zhiming; Shi, Pei-Yong. "Membrane Topology and Function of Dengue Virus NS2A Protein."  J. Virol. 87, 4609-4622 (2013).

Assembly members:
entity, polymer, 28 residues, 2981.647 Da.

Natural source:   Common Name: Dengue virus   Taxonomy ID: 12637   Superkingdom: Viruses   Kingdom: not available   Genus/species: Flavivirus Dengue virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: DIGMGVTYLALLAAFKVRPT FAAGLLLR

Data sets:
Data typeCount
13C chemical shifts26
15N chemical shifts26
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1trans-membrane domain of the NS2A1

Entities:

Entity 1, trans-membrane domain of the NS2A 28 residues - 2981.647 Da.

1   ASPILEGLYMETGLYVALTHRTYRLEUALA
2   LEULEUALAALAPHELYSVALARGPROTHR
3   PHEALAALAGLYLEULEULEUARG

Samples:

sample_1: entity 3 mg/mL; chloroform, [U-2H], 44.4%; methanol, [U-2H], 44.4%; H2O 11.2%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAD36758 BAD36759 BAD36760 BAD42415 BAD42416
EMBL CAD31751 CAI92123 CAR65133 CAR65134 CAR65135
GB AAA42941 AAA42942 AAA42962 AAA66406 AAA73185
PRF 1301272A
REF NP_056776 NP_739585
SP P07564 P12823 P14337 P14340 P29990