BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18816

Title: Solution structure of the tenth complement type repeat of human megalin   PubMed: 23275343

Authors: Dagil, Robert; Kragelund, Birthe

Citation: Dagil, Robert; Nykjaer, Charlotte; Bonvin, Anders; Kragelund, Alexandre M J J. "Gentamicin binds to the megalin receptor as a competitive inhibitor using the common ligand binding motif of complement type repeats: insight from the nmr structure of the 10th complement type repeat domain alone and in complex with gentamicin."  J. Biol. Chem. 288, 4424-4435 (2013).

Assembly members:
CR10, polymer, 52 residues, 5045.292 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
CR10: THAPASCLDTQYTCDNHQCI SKNWVCDTDNDCGDGSDEKN CNSTETHHHHHH

Data sets:
Data typeCount
13C chemical shifts164
15N chemical shifts44
1H chemical shifts243

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CR101
2Calcium ion2

Entities:

Entity 1, CR10 52 residues - 5045.292 Da.

1   THRHISALAPROALASERCYSLEUASPTHR
2   GLNTYRTHRCYSASPASNHISGLNCYSILE
3   SERLYSASNTRPVALCYSASPTHRASPASN
4   ASPCYSGLYASPGLYSERASPGLULYSASN
5   CYSASNSERTHRGLUTHRHISHISHISHIS
6   HISHIS

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CR10, [U-100% 13C; U-100% 15N], 0.6 mM; sodium chloride 100 mM; calcium chloride 50 mM; TRIS 50 mM; DSS 0.1 mM; sodium azide 0.1%; H2O 90%; D2O 10%

sample_2: CR10, [U-100% 13C; U-100% 15N], 0.6 mM; calcium chloride 50 mM; sodium chloride 100 mM; TRIS 50 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 100%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSY aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

Analysis v2.2, CCPN - chemical shift assignment, peak picking

ARIA, Linge, O, . - refinement, structure solution

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 800 MHz
  • Bruker Avance 800 MHz

Related Database Links:

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