BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18806

Title: Solution Structure of Miz-1 zinc fingers 5 to 7   PubMed: 23975355

Authors: Bernard, David; Bedard, Mikael; Bilodeau, Josee; Lavigne, Pierre

Citation: Bernard, David; Bedard, Mikael; Bilodeau, Josee; Lavigne, Pierre. "Structural and dynamical characterization of the Miz-1 zinc fingers 5-8 by solution-state NMR."  J. Biomol. NMR 57, 103-116 (2013).

Assembly members:
Miz58, polymer, 112 residues, 12953.9494 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Miz58: MKPYQCDYCGRSFSDPTSKM RHLETHDTDKEHKCPHCDKK FNQVGNLKAHLKIHIADGPL KCRECGKQFTTSGNLKRHLR IHSGEKPYVCIHCQRQFADP GALQRHVRIHTG

Data sets:
Data typeCount
13C chemical shifts429
15N chemical shifts98
1H chemical shifts659

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Miz581
2Zinc ions2

Entities:

Entity 1, Miz58 112 residues - 12953.9494 Da.

1   METLYSPROTYRGLNCYSASPTYRCYSGLY
2   ARGSERPHESERASPPROTHRSERLYSMET
3   ARGHISLEUGLUTHRHISASPTHRASPLYS
4   GLUHISLYSCYSPROHISCYSASPLYSLYS
5   PHEASNGLNVALGLYASNLEULYSALAHIS
6   LEULYSILEHISILEALAASPGLYPROLEU
7   LYSCYSARGGLUCYSGLYLYSGLNPHETHR
8   THRSERGLYASNLEULYSARGHISLEUARG
9   ILEHISSERGLYGLULYSPROTYRVALCYS
10   ILEHISCYSGLNARGGLNPHEALAASPPRO
11   GLYALALEUGLNARGHISVALARGILEHIS
12   THRGLY

Entity 2, Zinc ions - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Miz58, [U-13C; U-15N], 0.65 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
Expt_5 (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - structure calculations, refinement

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculations, refinement

ANALYSIS v2.1, CCPN - chemical shift assignment, data analysis

DANGLE v1.1, CCPN - data analysis

NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

UniProt Q13105