BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18773

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Staphylococcal Complement Inhibitor SCIN-B   PubMed: 23233676

Authors: Garcia, Brandon; Laity, John

Citation: Garcia, Brandon; Summers, Brady; Ramyar, Kasra; Tzekou, Apostolia; Lin, Zhuoer; Ricklin, Daniel; Lambris, John; Laity, John; Geisbrecht, Brian. "A Structurally Dynamic N-terminal Helix Is a Key Functional Determinant in Staphylococcal Complement Inhibitor (SCIN) Proteins."  J. Biol. Chem. 288, 2870-2881 (2013).

Assembly members:
SCIN-B, polymer, 85 residues, Formula weight is not available

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 158878   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SCIN-B: SSLDKYLTESQFHDKRIAEE LRTLLNKSNVYALAAGSLNP YYKRTIMMNEYRAKAALKKN DFVSMADAKVALEKIYKEID EIINR

Data sets:
Data typeCount
13C chemical shifts242
15N chemical shifts82
1H chemical shifts82
heteronuclear NOE values63
T1 relaxation values64
T2 relaxation values85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SCIN-B1

Entities:

Entity 1, SCIN-B 85 residues - Formula weight is not available

1   SERSERLEUASPLYSTYRLEUTHRGLUSER
2   GLNPHEHISASPLYSARGILEALAGLUGLU
3   LEUARGTHRLEULEUASNLYSSERASNVAL
4   TYRALALEUALAALAGLYSERLEUASNPRO
5   TYRTYRLYSARGTHRILEMETMETASNGLU
6   TYRARGALALYSALAALALEULYSLYSASN
7   ASPPHEVALSERMETALAASPALALYSVAL
8   ALALEUGLULYSILETYRLYSGLUILEASP
9   GLUILEILEASNARG

Samples:

sample_1: SCIN-B, [U-13C; U-15N], 800 uM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.0 M; pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CA)CO(CA)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, collection, data analysis, peak picking, processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB42254 BAB57321 BAB94906 BAF67342 BAF78032
EMBL CAG42867 CBI49030 CBX34394 CCW21394 CDP40783
GB AAW36548 ABD22046 ABD30229 ABQ49016 ABR52094
REF WP_000669532 WP_000669536 WP_000669537 WP_001633847 WP_023913704