BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18772

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Staphylococcal Complement Inhibitor SCIN-A   PubMed: 23233676

Authors: Garcia, Brandon; Laity, John

Citation: Garcia, Brandon; Summers, Brady; Ramyar, Kasra; Tzekou, Apostolia; Lin, Zhuoer; Ricklin, Daniel; Lambris, John; Laity, John; Geisbrecht, Brian. "A Structurally Dynamic N-terminal Helix Is a Key Functional Determinant in Staphylococcal Complement Inhibitor (SCIN) Proteins."  J. Biol. Chem. 288, 2870-2881 (2013).

Assembly members:
SCIN-A, polymer, 85 residues, Formula weight is not available

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 158878   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SCIN-A: STSLPTSNEYQNEKLANELK SLLDELNVNELATGSLNTYY KRTIKISGQKAMYALKSKDF KKMSEAKYQLQKIYNEIDEA LKSKY

Data sets:
Data typeCount
13C chemical shifts240
15N chemical shifts78
1H chemical shifts78
heteronuclear NOE values84
T1 relaxation values63
T2 relaxation values85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SCIN-A1

Entities:

Entity 1, SCIN-A 85 residues - Formula weight is not available

1   SERTHRSERLEUPROTHRSERASNGLUTYR
2   GLNASNGLULYSLEUALAASNGLULEULYS
3   SERLEULEUASPGLULEUASNVALASNGLU
4   LEUALATHRGLYSERLEUASNTHRTYRTYR
5   LYSARGTHRILELYSILESERGLYGLNLYS
6   ALAMETTYRALALEULYSSERLYSASPPHE
7   LYSLYSMETSERGLUALALYSTYRGLNLEU
8   GLNLYSILETYRASNGLUILEASPGLUALA
9   LEULYSSERLYSTYR

Samples:

sample_1: SCIN-A, [U-13C; U-15N], 800 uM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.0 M; pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CA)CO(CA)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, collection, data analysis, peak picking, processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB43028 BAB58104 BAB95749 BAF68148 BAF78810
EMBL CAG41021 CAG43672 CBI49810 CBX35157 CCE59622
GB AAL82379 ABD22701 ABD31213 ABF73224 ABQ49783
REF NP_803404 NP_835579 WP_000702262 WP_000702263 WP_001591359
SP A6QIG6 A7X482 Q2FFF8 Q2FWV6 Q6G7Z2