BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18769

Title: Structural study of NS2(32-57) GBVB protein   PubMed: 24741107

Authors: Montserret, Roland; Penin, Francois; Martin, Annette

Citation: Boukadida, Celia; Marnata, Caroline; Montserret, Roland; Cohen, Lisette; Blumen, Brigitte; Gouttenoire, Jerome; Moradpour, Darius; Penin, Francois; Martin, Annette. "NS2 proteins of GB virus B and hepatitis C virus share common protease activities and membrane topologies."  J. Virol. ., .-. (2014).

Assembly members:
NS2_32-57_GBVB, polymer, 26 residues, 3411.867 Da.

Natural source:   Common Name: Hepatitis GB virus B   Taxonomy ID: 39113   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
NS2_32-57_GBVB: RSALVNSYLWQRWENWFWNV TLRPER

Data sets:
Data typeCount
13C chemical shifts108
1H chemical shifts205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NS2_32-57_GBVB1

Entities:

Entity 1, NS2_32-57_GBVB 26 residues - 3411.867 Da.

1   ARGSERALALEUVALASNSERTYRLEUTRP
2   GLNARGTRPGLUASNTRPPHETRPASNVAL
3   THRLEUARGPROGLUARG

Samples:

sample_1: NS2_32-57_GBVB, [U-99% 2H], 2 ± 0.1 mM; TFE 50 ± 1 % v/v; H2O 50 ± 1 % v/v; DSS 0.2 ± 0.05 uM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz

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