BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18766

Title: Spatial stucture of PI-AnmTX Ugr 9a-1   PubMed: 23801332

Authors: Mineev, Konstantin; Arseniev, Alexander

Citation: Osmakov, Dmitry; Kozlov, Sergey; Andreev, Yaroslav; Koshelev, Sergey; Sanamyan, Nadezhda; Sanamyan, Karen; Dyachenko, Igor; Bondarenko, Dmitry; Murashev, Arkadii; Mineev, Konstantin; Arseniev, Alexander; Grishin, Eugene. "Sea anemone peptide with uncommon -hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity."  J. Biol. Chem. 288, 23116-23127 (2013).

Assembly members:
UGTX, polymer, 29 residues, 3142.515 Da.

Natural source:   Common Name: sea anemones   Taxonomy ID: 45620   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Urticina grebelnyi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UGTX: ISIDPPCRFCYHRDGSGNCV YDAYGCGAV

Data sets:
Data typeCount
13C chemical shifts77
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PI-AnmTX Ugr 9a-11

Entities:

Entity 1, PI-AnmTX Ugr 9a-1 29 residues - 3142.515 Da.

1   ILESERILEASPPROPROCYSARGPHECYS
2   TYRHISARGASPGLYSERGLYASNCYSVAL
3   TYRASPALATYRGLYCYSGLYALAVAL

Samples:

sample_1: UGTX 2 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0.3, Cornilescu, Delaglio and Bax - collection, processing

CARA v1.9.4, Keller and Wuthrich - chemical shift assignment, data analysis

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - data analysis, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

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