BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18736

Title: Solution structure of a Novel Alpha-Conotoxin TxIB   PubMed: 23184959

Authors: Luo, Sulan; Zhangsun, Dongting; Wu, Yong; Zhu, Xiaopeng; Hu, Yuanyan; McIntyre, Melissa; Christensen, Sean; Akcan, Muharrem; Craik, David J.; McIntosh, J. Michael

Citation: Luo, Sulan; Zhangsun, Dongting; Wu, Yong; Zhu, Xiaopeng; Hu, Yuanyan; McIntyre, Melissa; Christensen, Sean; Akcan, Muharrem; Craik, David; McIntosh, J. Michael. "Characterization of a Novel alpha-Conotoxin from Conus textile That Selectively Targets alpha6/alpha3beta2beta3 Nicotinic Acetylcholine Receptors"  J. Biol. Chem. 288, 894-902 (2013).

Assembly members:
Conotoxin_TxIB, polymer, 17 residues, 1746.020 Da.

Natural source:   Common Name: Cloth-of-gold cone   Taxonomy ID: 6494   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus Textile

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Conotoxin_TxIB: GCCSDPPCRNKHPDLCX

Data sets:
Data typeCount
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Conotoxin_TxIB1

Entities:

Entity 1, Conotoxin_TxIB 17 residues - 1746.020 Da.

1   GLYCYSCYSSERASPPROPROCYSARGASN
2   LYSHISPROASPLEUCYSNH2

Samples:

sample_1: Conotoxin_TxIB 3 mg; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 3.5; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

MolProbity, Richardson - Structure validation

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CCM73829
SP K4RNX9