BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18731

Title: Solid-state NMR sequential assignments of the C-terminal oligomerization domain of human C4b-binding protein   PubMed: 23138856

Authors: Luckgei, Nina; Habenstein, Birgit; Ravotti, Francesco; Megy, Simon; Penin, Francois; Marchand, Jean-Baptiste; Hill, Fergal; Boeckmann, Anja; Meier, Beat

Citation: Luckgei, Nina; Habenstein, Birgit; Ravotti, Francesco; Megy, Simon; Penin, Francois; Marchand, Jean-Baptiste; Hill, Fergal; Bockmann, Anja; Meier, Beat. "Solid-state NMR sequential assignments of the C-terminal oligomerization domain of human C4b-binding protein."  Biomol. NMR Assignments ., .-. (2012).

Assembly members:
IMX77, polymer, 65 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IMX77: ASMNHKGSETPEGCEQVLTG KRLMQCLPNPEDVKMALEVY KLSLEIEQLELQRDSARQST LDKEL

Data sets:
Data typeCount
13C chemical shifts187
15N chemical shifts43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IMX771

Entities:

Entity 1, IMX77 65 residues - Formula weight is not available

The sequence starts with the number minus 8 and the position '0' is skipped

1   ALASERMETASNHISLYSGLYSERGLUTHR
2   PROGLUGLYCYSGLUGLNVALLEUTHRGLY
3   LYSARGLEUMETGLNCYSLEUPROASNPRO
4   GLUASPVALLYSMETALALEUGLUVALTYR
5   LYSLEUSERLEUGLUILEGLUGLNLEUGLU
6   LEUGLNARGASPSERALAARGGLNSERTHR
7   LEUASPLYSGLULEU

Samples:

sample_IMX77: IMX77, [U-100% 13C; U-100% 15N], 10 ± 1 mg; TRIS 50 ± 5 mM; ethanol 20 ± 2 %; MPD 15 ± 2 %

sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 263 K

Experiments:

NameSampleSample stateSample conditions
DARRsample_IMX77isotropicsample_conditions_1
NCACBsample_IMX77isotropicsample_conditions_1
NCACXsample_IMX77isotropicsample_conditions_1
CANCOsample_IMX77isotropicsample_conditions_1
NCOCAsample_IMX77isotropicsample_conditions_1
NCOsample_IMX77isotropicsample_conditions_1
NCAsample_IMX77isotropicsample_conditions_1
CCCsample_IMX77isotropicsample_conditions_1

Software:

NMR-STAR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAG35982 BAG64750 BAG64753 BAJ20441
EMBL CAA26617
GB AAA36506 AAA36507 AAH22312 ABM82947 ABM86139
REF NP_000706 XP_002809571 XP_002832590 XP_003822948 XP_004028360
SP P04003