BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18678

Title: NMR structure of the N-terminal RNA Binding domain 1 (RRM1) of the protein RBM10 from Homo sapiens

Authors: Serrano, Pedro; Geralt, Michael; Dutta, Samit; Wuthrich, Kurt

Citation: Serrano, Pedro; Wuthrich, Kurt. "NMR structure of the N-terminal RNA Binding domain 1 (RRM1) of the protein RBM10 from Homo sapiens"  Not known ., .-..

Assembly members:
entity, polymer, 91 residues, 10479.927 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: SNIVMLRMLPQAATEDDIRG QLQSHGVQAREVRLMRNKSS GQSRGFAFVEFSHLQDATRW MEANQHSLNILGQKVSMHYS DPKPKINEDWL

Data sets:
Data typeCount
13C chemical shifts368
15N chemical shifts101
1H chemical shifts627

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM1-RBM101

Entities:

Entity 1, RRM1-RBM10 91 residues - 10479.927 Da.

1   SERASNILEVALMETLEUARGMETLEUPRO
2   GLNALAALATHRGLUASPASPILEARGGLY
3   GLNLEUGLNSERHISGLYVALGLNALAARG
4   GLUVALARGLEUMETARGASNLYSSERSER
5   GLYGLNSERARGGLYPHEALAPHEVALGLU
6   PHESERHISLEUGLNASPALATHRARGTRP
7   METGLUALAASNGLNHISSERLEUASNILE
8   LEUGLYGLNLYSVALSERMETHISTYRSER
9   ASPPROLYSPROLYSILEASNGLUASPTRP
10   LEU

Samples:

sample_1: RRM1-RBM10, [U-98% 13C; U-98% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.220 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
APSY 4D-HACANHsample_1isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
APSY 5D-HACACONHsample_1isotropicsample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC65490
GB EHB02419 ERE65732
REF XP_010993528 XP_012354033