BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18658

Title: NMR solution structure of peptide a2N(1-17) from Mus musculus V-ATPase   PubMed: 23288846

Authors: Dip, Phat Vinh; Gruber, Gerhard; Marshansky, Vladimir

Citation: Hosokawa, Hiroyuki; Dip, Phat Vinh; Merkulova, Maria; Bakulina, Anastasia; Zhuang, Zhenjie; Khatri, Ashok; Jian, Xiaoying; Keating, Shawn; Bueler, Stephanie; Rubinstein, John; Randazzo, Paul; Ausiello, Dennis; Gruber, Gerhard; Marshansky, Vladimir. "The N Termini of a-Subunit Isoforms Are Involved in Signaling between Vacuolar H+-ATPase (V-ATPase) and Cytohesin-2."  J. Biol. Chem. 288, 5896-5913 (2013).

Assembly members:
a2N(1-17), polymer, 17 residues, 1934.332 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
a2N(1-17): MGSLFRSESMCLAQLFL

Data sets:
Data typeCount
1H chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1peptide a2N(1-17) from Mus musculus V-ATPase1

Entities:

Entity 1, peptide a2N(1-17) from Mus musculus V-ATPase 17 residues - 1934.332 Da.

1   METGLYSERLEUPHEARGSERGLUSERMET
2   CYSLEUALAGLNLEUPHELEU

Samples:

sample_1: a2N(1-17) 2 mM; TFE, [U-99% 2H], 50%; sodium phosphate 25 mM; sodium chloride 300 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, chemical shift calculation, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA93007 BAE33017
EMBL CAA38968
GB AAA39336 AAF59921 AAI08992 AAI08993 AAI12906
REF NP_035726 NP_446227 XP_003420372 XP_004385053 XP_004668552
SP P15920