BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18651

Title: 13C and 15N chemical shifts for Shigella Flexneri MxiH Type three secretion system needle subunit   PubMed: 23555258

Authors: Demers, Jean-Philippe; Sgourakis, Nikolaos; Gupta, Rashmi; Loquet, Antoine; Giller, Karin; Riedel, Dietmar; Laube, Britta; Kolbe, Michael; Baker, David; Becker, Stefan; Lange, Adam

Citation: Demers, Jean-Philippe; Sgourakis, Nikolaos; Gupta, Rashmi; Loquet, Antoine; Giller, Karin; Riedel, Dietmar; Laube, Britta; Kolbe, Michael; Baker, David; Becker, Stefan; Lange, Adam. "The Common Structural Architecture of Shigella flexneri and Salmonella typhimurium Type Three Secretion Needles"  PLoS Pathog. 9, e1003245-e1003245 (2013).

Assembly members:
MxiH, polymer, 85 residues, 9389.3 Da.

Natural source:   Common Name: Shigella flexneri   Taxonomy ID: 424719   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shigella flexneri

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MxiH: GHMSVTVPDKDWTLSSLSET FDDGTQTLQGQLTSALNALA ENPSNPQLLAEYQSKLSEYT LYRNAQSNTVKVIKDVDAAI IQNFR

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MxiH needle1

Entities:

Entity 1, MxiH needle 85 residues - 9389.3 Da.

The sequence employed in this study is found in S. flexneri serotype 6. After protease cleavage of the N-terminal His tag, the released MxiH protein contains the non-native N-terminal residues glycine and histidine.

1   GLYHISMETSERVALTHRVALPROASPLYS
2   ASPTRPTHRLEUSERSERLEUSERGLUTHR
3   PHEASPASPGLYTHRGLNTHRLEUGLNGLY
4   GLNLEUTHRSERALALEUASNALALEUALA
5   GLUASNPROSERASNPROGLNLEULEUALA
6   GLUTYRGLNSERLYSLEUSERGLUTYRTHR
7   LEUTYRARGASNALAGLNSERASNTHRVAL
8   LYSVALILELYSASPVALASPALAALAILE
9   ILEGLNASNPHEARG

Samples:

sample_1: MxiH, [U-100% 13C; U-100% 15N], 20 mg

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
13C-13C (PDSD)sample_1solidsample_conditions_1
15N-13C (Specific-CP)sample_1solidsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

UNP Q6XVY0_SHIFL
PDB
GB AAP79000 ACD06130 KHQ70608 KIZ60406
REF WP_012421721 WP_032324888 WP_039064401 WP_046891853 WP_047204460