BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18648

Title: APPTM V44M   PubMed: 24390130

Authors: Chen, Wen; Wang, Chunyu

Citation: Chen, Wen; Gamache, Eric; Rosenman, David; Xie, Jian; Lopez, Maria; Li, Yue-Ming; Wang, Chunyu. "Familial Alzheimer's mutations within APPTM increase A42 production by enhancing accessibility of -cleavage site."  Nat. Commun. 5, 3037-3037 (2014).

Assembly members:
V44M monomer, polymer, 32 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
V44M monomer: GAMAKGAIIGLMVGGVVIAT MIVITLVMLKKK

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts30
1H chemical shifts252

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1V44M chain, 11
2V44M chain, 21

Entities:

Entity 1, V44M chain, 1 32 residues - Formula weight is not available

1   GLYALAMETALALYSGLYALAILEILEGLY
2   LEUMETVALGLYGLYVALVALILEALATHR
3   METILEVALILETHRLEUVALMETLEULYS
4   LYSLYS

Samples:

15N_V44M: APPTM V44M dimer, [U-100% 13C; U-100% 15N], mM; potassium chloride 0.5 mM; sodium chloride 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_V44Misotropicsample_conditions_1
2D 1H-13C HSQC15N_V44Misotropicsample_conditions_1
3D C(CO)NH15N_V44Misotropicsample_conditions_1
3D HNCO15N_V44Misotropicsample_conditions_1
3D HNCACB15N_V44Misotropicsample_conditions_1
3D HBHA(CO)NH15N_V44Misotropicsample_conditions_1
3D H(CCO)NH15N_V44Misotropicsample_conditions_1
3D HCCH-TOCSY15N_V44Misotropicsample_conditions_1
3D 1H-15N TOCSY15N_V44Misotropicsample_conditions_1

Software:

SPARKY, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

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