BMRB Entry 18628

Title:
1H, 13C, and 15N Backbone and Sidechain Resonance Assignments for Cyclophilin A from Geobacillus Kaustophilus
Deposition date:
2012-07-31
Original release date:
2013-02-12
Authors:
Holliday, Michael; Isern, Nancy; Geoffrey, Armstrong; Zhang, Fengli; Eisenmesser, Elan
Citation:

Citation: Holliday, Michael; Zhang, Fengli; Isern, Nancy; Armstrong, Geoffrey; Eisenmesser, Elan. "1H, 13C, and 15N backbone and side chain resonance assignments of thermophilic Geobacillus kaustophilus cyclophilin-A."  Biomol. NMR Assignments ., .-. (2012).
PubMed: 23138858

Assembly members:

Assembly members:
gCypA, polymer, 146 residues, 15975.0193 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1462   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus kaustophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet15b

Data typeCount
13C chemical shifts586
15N chemical shifts145
1H chemical shifts920

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gCypA1

Entities:

Entity 1, gCypA 146 residues - 15975.0193 Da.

1   METALALYSLYSGLYTYRILELEUMETGLU
2   ASNGLYGLYLYSILEGLUPHEGLULEUPHE
3   PROASNGLUALAPROVALTHRVALALAASN
4   PHEGLULYSLEUALAASNGLUGLYPHETYR
5   ASNGLYLEUTHRPHEHISARGVALILEPRO
6   GLYPHEVALSERGLNGLYGLYCYSPROARG
7   GLYASNGLYTHRGLYASPALAGLYTYRTHR
8   ILEPROCYSGLUTHRASPASNASNPROHIS
9   ARGHISVALTHRGLYALAMETSERMETALA
10   HISARGGLYARGASPTHRGLYSERCYSGLN
11   PHEPHEILEVALHISGLUPROGLNPROHIS
12   LEUASPGLYVALHISTHRVALPHEGLYGLN
13   VALTHRSERGLYMETASPVALVALARGTHR
14   METLYSASNGLYASPVALMETLYSGLUVAL
15   LYSVALPHEASPGLUPRO

Samples:

13C15N: GeoCyp, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 93%; D2O 7%; DTT 1 mM; sodium phosphate 50 mM

Set1: pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQC13C15NisotropicSet1
3D HNCACB13C15NisotropicSet1
3D CBCA(CO)NH13C15NisotropicSet1
3D C(CO)NH13C15NisotropicSet1
3D 1H-15N NOESY13C15NisotropicSet1
3D 1H-13C NOESY13C15NisotropicSet1
2D 1H-13C HSQC/HMQC13C15NisotropicSet1
3D 1H-15N TOCSY13C15NisotropicSet1
3D HCCH-TOCSY13C15NisotropicSet1
3D HNCACB13C15NisotropicSet1
3D 1H-13C NOESY13C15NisotropicSet1
hbCBcgcdceHE13C15NisotropicSet1
3D HNCO13C15NisotropicSet1
3D 1H-13C NOESY13C15NisotropicSet1

Software:

ANALYSIS v2.1, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 720 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB TmpAcc 25336
PDB
DBJ BAD76583 GAD12357 GAJ58355
GB ABO67576 ACX77066 ADI26232 ADU94680 AEV19889
REF WP_008879707 WP_011231780 WP_012820689 WP_013144839 WP_025948556

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks