BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18625

Title: NMR Structure of the Self-Complementary 10 mer DNA Oligonucleotide 5'-GGATATATCC-3'.   PubMed: 23355266

Authors: Rettig, Michael; Germann, Markus; Wilson, W. David; Wang, Shuo

Citation: Rettig, Michael; Germann, Markus; Wang, Shuo; Wilson, W. David. "Molecular basis for sequence-dependent induced DNA bending."  Chembiochem 14, 323-331 (2013).

Assembly members:
DNA_(5'-D(*GP*GP*AP*TP*AP*TP*AP*TP*CP*C)-3')_, polymer, 10 residues, 3044.042 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA_(5'-D(*GP*GP*AP*TP*AP*TP*AP*TP*CP*C)-3')_: GGATATATCC

Data sets:
Data typeCount
1H chemical shifts200

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GGATATATCC, 11
2GGATATATCC, 21

Entities:

Entity 1, GGATATATCC, 1 10 residues - 3044.042 Da.

1   DGDGDADTDADTDADTDCDC

Samples:

sample_1_D2O: GGATATATCC 0.9 mM

sample_1_H2O: GGATATATCC 0.9 mM

sample_1_Pf1: GGATATATCC 1.2 mM; Pf1 phage 11 mg/mL

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1_D2Oisotropicsample_conditions_1
2D 1H-1H NOESYsample_1_H2Oisotropicsample_conditions_1
2D DQF-COSYsample_1_D2Oisotropicsample_conditions_1
2D 1H-1H NOESYsample_1_Pf1anisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1_Pf1anisotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1_Pf1anisotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links: