BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18616

Title: Relaxation rates for accurate spectral density mapping for unbiased analysis of nucleic acid motions probed by carbon-13 NMR relaxation   PubMed: 27003380

Authors: Kaderavek, Pavel; Zapletal, Vojtech; Fiala, Radovan; Sklenar, Vladimir; Zidek, Lukas

Citation: Kaderavek, Pavel; Zapletal, Vojtech; Fiala, Radovan; Srb, Pavel; Padrta, Petr; Precechtelova, Jana Pavlikova; Soltesova, Maria; Kowalewski, Jozef; Widmalm, Goran; Chmelik, Josef; Sklenar, Vladimir; Zidek, Lukas. "Spectral density mapping at multiple magnetic fields suitable for (13)C NMR relaxation studies"  J. Magn. Reson. 266, 23-40 (2016).

Assembly members:
UUCG_tetraloop, polymer, 14 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
UUCG_tetraloop: GGCACUUCGGUGCC

Data typeCount
T1 relaxation values39
T1rho relaxation values39
dipole-CSA cross correlation relaxation values73
heteronuclear NOE values52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1UUCG teraloop1

Entities:

Entity 1, UUCG teraloop 14 residues - Formula weight is not available

1   GGCACUUCGG
2   UGCC

Samples:

sample_1: UUCG tetraloop, [U-13C; U-15N], 3.0 mM; EDTA 0.2 mM; sodium phosphate 10 mM; sodium azide 0.05%

sample_conditions_1: pH*: 6.7; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D R1 13C,1H correlationsample_1isotropicsample_conditions_1
2D R1rho 13C,1H correlationsample_1isotropicsample_conditions_1
2D NOE 13C,1H correlationsample_1isotropicsample_conditions_1
2D constant-time 13C(coupled),1H correlationsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian Unity 400 MHz
  • Varian Unity 500 MHz
  • Varian Unity 600 MHz

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