BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18589

Title: NMR solution structure of Cbp2   PubMed: 23325851

Authors: Kenchappa, Chandra; Heidarsson, Petur; Garrett, Roger; Poulsen, Flemming

Citation: Kenchappa, Chandra; Heidarsson, Petur; Kragelund, Birthe; Garrett, Roger; Poulsen, Flemming. "Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2."  Nucleic Acids Res. 41, 3424-3435 (2013).

Assembly members:
Cbp2, polymer, 105 residues, 12415 Da.

Natural source:   Common Name: Hyperthermus butylicus   Taxonomy ID: 54248   Superkingdom: Archaea   Kingdom: not available   Genus/species: Hyperthermus butylicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cbp2: MPSVNDSLDIVEKLYKDGVP VKEIAKRSNNSMSTVYKALE KLEAMGRIKRRKGRYRQHRR LTEEELATIRELYLKGATVY EIARQLGRPESTIYYALKKL GLKLE

Data sets:
Data typeCount
13C chemical shifts395
15N chemical shifts89
1H chemical shifts589

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cbp21

Entities:

Entity 1, Cbp2 105 residues - 12415 Da.

1   METPROSERVALASNASPSERLEUASPILE
2   VALGLULYSLEUTYRLYSASPGLYVALPRO
3   VALLYSGLUILEALALYSARGSERASNASN
4   SERMETSERTHRVALTYRLYSALALEUGLU
5   LYSLEUGLUALAMETGLYARGILELYSARG
6   ARGLYSGLYARGTYRARGGLNHISARGARG
7   LEUTHRGLUGLUGLULEUALATHRILEARG
8   GLULEUTYRLEULYSGLYALATHRVALTYR
9   GLUILEALAARGGLNLEUGLYARGPROGLU
10   SERTHRILETYRTYRALALEULYSLYSLEU
11   GLYLEULYSLEUGLU

Samples:

sample_1: Cbp2, [U-100% 13C; U-100% 15N], 0.5-1 mM; potassium chloride, none, 10 mM; potassium dihydrogen phosphate, none, 20 mM; sodium azide, none, 0.02%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

VNMRJ, Varian - collection

CCPN_Analysis, CCPN - chemical shift assignment, peak picking

PSVS, Bhattacharya and Montelione - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB ABM80833
REF WP_011822151