BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18586

Title: Solution structures of Miz-1 zinc fingers 8 to 10   PubMed: 22986688

Authors: Bedard, Mikael; Maltais, Loika; Beaulieu, Marie-Eve; Bernard, David; Lavigne, Pierre

Citation: Bedard, Mikael; Maltais, Loika; Beaulieu, Marie-Eve; Bilodeau, Josee; Bernard, David; Lavigne, Pierre. "NMR structure note: solution structure of human Miz-1 zinc fingers 8 to 10."  J. Biomol. NMR 54, 317-323 (2012).

Assembly members:
Miz8-10, polymer, 83 residues, 9512.0695 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Miz8-10: MKPYVCIHCQRQFADPGALQ RHVRIHTGEKPCQCVMCGKA FTQASSLIAHVRQHTGEKPY VCERCGKRFVQSSQLANHIR HHD

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts77
1H chemical shifts484

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Miz8-101
2ZINC ION2

Entities:

Entity 1, Miz8-10 83 residues - 9512.0695 Da.

1   METLYSPROTYRVALCYSILEHISCYSGLN
2   ARGGLNPHEALAASPPROGLYALALEUGLN
3   ARGHISVALARGILEHISTHRGLYGLULYS
4   PROCYSGLNCYSVALMETCYSGLYLYSALA
5   PHETHRGLNALASERSERLEUILEALAHIS
6   VALARGGLNHISTHRGLYGLULYSPROTYR
7   VALCYSGLUARGCYSGLYLYSARGPHEVAL
8   GLNSERSERGLNLEUALAASNHISILEARG
9   HISHISASP

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample-Miz8-10: Miz8-10, [U-13C; U-15N], 0.75 – 1.00 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample-Miz8-10isotropicsample_conditions_1
2D 1H-13C HSQCsample-Miz8-10isotropicsample_conditions_1
3D CBCA(CO)NHsample-Miz8-10isotropicsample_conditions_1
3D HNCACBsample-Miz8-10isotropicsample_conditions_1
3D HNCOsample-Miz8-10isotropicsample_conditions_1
3D C(CO)NHsample-Miz8-10isotropicsample_conditions_1
3D H(CCO)NHsample-Miz8-10isotropicsample_conditions_1
3D HCCH-TOCSYsample-Miz8-10isotropicsample_conditions_1
3D 1H-15N NOESYsample-Miz8-10isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample-Miz8-10isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample-Miz8-10isotropicsample_conditions_1
3D HNHAsample-Miz8-10isotropicsample_conditions_1

Software:

ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - refinement, structure calculation

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

ANALYSIS v2.1, CCPN - chemical shift assigment, data analysis

DANGLE v1.1, CCPN - data analysis

NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

UNP Q13105