BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18581

Title: Solution structure of gp78 CUE domain   PubMed: 23123110

Authors: Liu, Shan; Chen, Yinghua; Huang, Tao; Tarasov, Sergey; King, Aaren; Li, Jess; Weissman, Allan; Byrd, Robert; Das, Ranabir

Citation: Liu, Shan; Chen, Yinghua; Li, Jess; Huang, Tao; Tarasov, Sergey; King, Aaren; Weissman, Allan; Byrd, R. Andrew; Das, Ranabir. "Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains."  Structure 20, 2138-2150 (2012).

Assembly members:
entity, polymer, 52 residues, 5966.808 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: SNSQLNAMAHQIQEMFPQVP YHLVLQDLQLTRSVEITTDN ILEGRIQVPFPT

Data sets:
Data typeCount
13C chemical shifts231
15N chemical shifts53
1H chemical shifts378

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gp78 CUE domain1

Entities:

Entity 1, gp78 CUE domain 52 residues - 5966.808 Da.

1   SERASNSERGLNLEUASNALAMETALAHIS
2   GLNILEGLNGLUMETPHEPROGLNVALPRO
3   TYRHISLEUVALLEUGLNASPLEUGLNLEU
4   THRARGSERVALGLUILETHRTHRASPASN
5   ILELEUGLUGLYARGILEGLNVALPROPHE
6   PROTHR

Samples:

sample_1: TRIS 50 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker INOVA 600 MHz
  • Bruker INOVA 700 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE01277 BAE34049 BAE41974 BAK63135
GB AAD56721 AAD56722 AAH03256 AAH17043 AAH34538
REF NP_001039439 NP_001135 NP_001267243 NP_035917 XP_001091030
SP Q9R049 Q9UKV5
TPG DAA20037