BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18551

Title: Solution NMR structure of C2H2-type Zinc-fingers 4 and 5 from human Insulinoma-associated protein 1 (fragment 424-497), Northeast Structural Genomics Consortium Target HR7614B.

Authors: Yang, Yunhuang; Ramelot, Theresa; Cort, John; Shastry, Ritu; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Kennedy, Michael

Citation: Yang, Yunhuang; Ramelot, Theresa; Cort, John; Shastry, Ritu; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Kennedy, Michael. "Solution NMR structure of C2H2-type Zinc-fingers 4 and 5 from human Insulinoma-associated protein 1 (fragment 424-497), Northeast Structural Genomics Consortium Target HR7614B."  To be published ., .-..

Assembly members:
HR7614B, polymer, 85 residues, 9350.260 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HR7614B: MGHHHHHHSHMGDGEGAGVL GLSASAECHLCPVCGESFAS KGAQERHLRLLHAAQVFPCK YCPATFYSSPGLTRHINKCH PSENR

Data typeCount
13C chemical shifts307
15N chemical shifts79
1H chemical shifts474

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C2H2-type Zinc-fingers 4 and 5 from human Insulinoma-associated protein 1 (fragment 424-497)1
2Zinc ion, 12
3Zinc ion, 22

Entities:

Entity 1, C2H2-type Zinc-fingers 4 and 5 from human Insulinoma-associated protein 1 (fragment 424-497) 85 residues - 9350.260 Da.

1   METGLYHISHISHISHISHISHISSERHIS
2   METGLYASPGLYGLUGLYALAGLYVALLEU
3   GLYLEUSERALASERALAGLUCYSHISLEU
4   CYSPROVALCYSGLYGLUSERPHEALASER
5   LYSGLYALAGLNGLUARGHISLEUARGLEU
6   LEUHISALAALAGLNVALPHEPROCYSLYS
7   TYRCYSPROALATHRPHETYRSERSERPRO
8   GLYLEUTHRARGHISILEASNLYSCYSHIS
9   PROSERGLUASNARG

Entity 2, Zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

NC_sample: HR7614B.021, [U-100% 13C; U-100% 15N], 0.69 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.02%; Tris-HCl pH 7.5 10 mM

NC5_sample: HR7614B.023, [U-5% 13C; U-100% 15N], 1.25 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.02%; Tris-HCl pH 7.5 10 mM

NC_D2O_sample: HR7614B.023, [U-5% 13C; U-100% 15N], 1.25 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.02%; Tris-HCl pH 7.5 10 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNC_sampleisotropicsample_conditions_1
2D 1H-13C HSQCNC_sampleisotropicsample_conditions_1
3D HNCONC_sampleisotropicsample_conditions_1
3D CBCA(CO)NHNC_sampleisotropicsample_conditions_1
3D HNCACBNC_sampleisotropicsample_conditions_1
3D 1H-13C NOESY aromaticNC_sampleisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticNC_sampleisotropicsample_conditions_1
3D C(CO)NHNC_sampleisotropicsample_conditions_1
3D HNCANC_sampleisotropicsample_conditions_1
3D HN(CO)CANC_sampleisotropicsample_conditions_1
3D H(CCO)NHNC_sampleisotropicsample_conditions_1
3D HCCH-TOCSYNC_sampleisotropicsample_conditions_1
3D HNHANC_sampleisotropicsample_conditions_1
4D CC-NOESYNC_D2O_sampleisotropicsample_conditions_1
2D 1H-13C HSQC_CT aliphaticNC5_sampleisotropicsample_conditions_1
2D 1H-13C HSQC_noCT aliphaticNC5_sampleisotropicsample_conditions_1
3D (H)CCH-TOCSYNC_D2O_sampleisotropicsample_conditions_1
3D NnoesyNC_sampleisotropicsample_conditions_1
3D 1H-13C NOESY NUSNC_sampleisotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis,refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - data analysis,chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis,peak picking,chemical shift assignment

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

SPARKY, Goddard - data analysis

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

PSVS, Bhattacharya, Montelione - structure validation

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA58680
REF NP_002187 XP_001091915 XP_001144246 XP_002830051 XP_003732871
SP Q01101