BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18544

Title: Chemical shift assignments of DsbA(C33S)/DsbB by solid-state NMR   PubMed: 23527473

Authors: Sperling, Lindsay; Tang, Ming; Berthold, Deborah; Nesbitt, Anna; Gennis, Robert; Rienstra, Chad

Citation: Sperling, Lindsay; Tang, Ming; Berthold, Deborah; Nesbitt, Anna; Gennis, Robert; Rienstra, Chad. "Solid-State NMR Study of a 41 kDa Membrane Protein Complex DsbA/DsbB."  J. Phys. Chem. B 117, 6052-6060 (2013).

Assembly members:
DsbA(C33S), polymer, 189 residues, Formula weight is not available
DsbB, polymer, 176 residues, Formula weight is not available

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DsbA(C33S): AQYEDGKQYTTLEKPVAGAP QVLEFFSFFCPHSYQFEEVL HISDNVKKKLPEGVKMTKYH VNFMGGDLGKDLTQAWAVAM ALGVEDKVTVPLFEGVQKTQ TIRSASDIRDVFINAGIKGE EYDAAWNSFVVKSLVAQQEK AAADVQLRGVPAMFVNGKYQ LNPQGMDTSNMDVFVQQYAD TVKYLSEKK
DsbB: MLRFLNQASQGRGAWLLMAF TALALELTALWFQHVMLLKP CVLCIYERVALFGVLGAALI GAIAPKTPLRYVAMVIWLYS AFRGVQLTYEHTMLQLYPSP FATCDFMVRFPEWLPLDKWV PQVFVASGDCAERQWDFLGL EMPQWLLGIFIAYLIVAVLV VISQPFKAKKRDLFGR

Data sets:
Data typeCount
13C chemical shifts268
15N chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbA(C33S)1
2DsbB2

Entities:

Entity 1, DsbA(C33S) 189 residues - Formula weight is not available

1   ALAGLNTYRGLUASPGLYLYSGLNTYRTHR
2   THRLEUGLULYSPROVALALAGLYALAPRO
3   GLNVALLEUGLUPHEPHESERPHEPHECYS
4   PROHISSERTYRGLNPHEGLUGLUVALLEU
5   HISILESERASPASNVALLYSLYSLYSLEU
6   PROGLUGLYVALLYSMETTHRLYSTYRHIS
7   VALASNPHEMETGLYGLYASPLEUGLYLYS
8   ASPLEUTHRGLNALATRPALAVALALAMET
9   ALALEUGLYVALGLUASPLYSVALTHRVAL
10   PROLEUPHEGLUGLYVALGLNLYSTHRGLN
11   THRILEARGSERALASERASPILEARGASP
12   VALPHEILEASNALAGLYILELYSGLYGLU
13   GLUTYRASPALAALATRPASNSERPHEVAL
14   VALLYSSERLEUVALALAGLNGLNGLULYS
15   ALAALAALAASPVALGLNLEUARGGLYVAL
16   PROALAMETPHEVALASNGLYLYSTYRGLN
17   LEUASNPROGLNGLYMETASPTHRSERASN
18   METASPVALPHEVALGLNGLNTYRALAASP
19   THRVALLYSTYRLEUSERGLULYSLYS

Entity 2, DsbB 176 residues - Formula weight is not available

1   METLEUARGPHELEUASNGLNALASERGLN
2   GLYARGGLYALATRPLEULEUMETALAPHE
3   THRALALEUALALEUGLULEUTHRALALEU
4   TRPPHEGLNHISVALMETLEULEULYSPRO
5   CYSVALLEUCYSILETYRGLUARGVALALA
6   LEUPHEGLYVALLEUGLYALAALALEUILE
7   GLYALAILEALAPROLYSTHRPROLEUARG
8   TYRVALALAMETVALILETRPLEUTYRSER
9   ALAPHEARGGLYVALGLNLEUTHRTYRGLU
10   HISTHRMETLEUGLNLEUTYRPROSERPRO
11   PHEALATHRCYSASPPHEMETVALARGPHE
12   PROGLUTRPLEUPROLEUASPLYSTRPVAL
13   PROGLNVALPHEVALALASERGLYASPCYS
14   ALAGLUARGGLNTRPASPPHELEUGLYLEU
15   GLUMETPROGLNTRPLEULEUGLYILEPHE
16   ILEALATYRLEUILEVALALAVALLEUVAL
17   VALILESERGLNPROPHELYSALALYSLYS
18   ARGASPLEUPHEGLYARG

Samples:

sample_DsbA(C33S)_DsbB: DsbA(C33S), [U-100% 13C; U-100% 15N], 6 mg; H2O 90%; D2O, [U-2D], 10%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 263 K

sample_conditions_2: pH: 7.4; pressure: 1 atm; temperature: 253 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_DsbA(C33S)_DsbBsolidsample_conditions_2
3D NCOCXsample_DsbA(C33S)_DsbBsolidsample_conditions_2
2D CCsample_DsbA(C33S)_DsbBsolidsample_conditions_2
2D TEDORsample_DsbA(C33S)_DsbBsolidsample_conditions_2

Software:

VNMRJ v3.1, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian VXRS 500 MHz

Related Database Links:

BMRB 16327 17710 18396 18543 15546 15966 17710 18395 18493
PDB
DBJ BAB38206 BAE77448 BAG79665 BAI27892 BAI33015 BAA07408 BAA36032 BAB35103 BAG76757 BAI24997
EMBL CAA44868 CAA56736 CAA90910 CAP78318 CAQ34212 CAP75720 CAQ31687 CAQ98064 CAR02574 CAR07527
GB AAA23715 AAB02995 AAC43519 AAC43520 AAC43521 AAA23711 AAB25233 AAC74269 AAG56036 AAN42789
REF NP_312810 NP_418297 NP_709659 WP_000725331 WP_000725332 NP_287424 NP_309707 NP_415703 NP_707082 NP_753538
SP P0A4L5 P0A4L6 P0AEG4 P0AEG5 P52235 A1AAA8 P0A6M2 P0A6M3 P59343 Q0T5L6
PIR H85696