BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18511

Title: Solution NMR Structure of Ig like domain (1277-1357) of Obscurin-like protein 1 from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8578D

Authors: Pulavarti, Surya V.S.R.K; Eletsky, Alex; Satyamoorthy, Bharatwaj; Sukumaran, Dinesh; Lee, Dan; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Szyperski, Thomas

Citation: Pulavarti, Surya V.S.R.K; Eletsky, Alex; Satyamoorthy, Bharatwaj; Sukumaran, Dinesh; Lee, Dan; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR Structure of Ig like domain (1277-1357) of Obscurin-like protein 1 from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8578D"  To be published ., .-..

Assembly members:
HR8578D, polymer, 84 residues, 8888.005 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HR8578D: SHMTRVRSTPGGDLELVVHL SGPGGPVRWYKDGERLASQG RVQLEQAGARQVLRVQGARS GDAGEYLCDAPQDSRIFLVS VEEP

Data sets:
Data typeCount
1H chemical shifts542
13C chemical shifts332
15N chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HR8578D1

Entities:

Entity 1, HR8578D 84 residues - 8888.005 Da.

1   SERHISMETTHRARGVALARGSERTHRPRO
2   GLYGLYASPLEUGLULEUVALVALHISLEU
3   SERGLYPROGLYGLYPROVALARGTRPTYR
4   LYSASPGLYGLUARGLEUALASERGLNGLY
5   ARGVALGLNLEUGLUGLNALAGLYALAARG
6   GLNVALLEUARGVALGLNGLYALAARGSER
7   GLYASPALAGLYGLUTYRLEUCYSASPALA
8   PROGLNASPSERARGILEPHELEUVALSER
9   VALGLUGLUPRO

Samples:

sample_1: HR8578D.005, [U-100% 13C; U-100% 15N], 0.56 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.02%; Tris-HCl pH 7.5 10 mM; H20 90%; D20 10%

sample_2: HR8578D.006, [U-5% 13C; U-100% 15N], 0.52 mM; NaCl 100 mM; DTT 5 mM; NaN3 0.02%; Tris-HCl pH 7.5 10 mM; H20 90%; D20 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC (CT-28 ms) aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC (CT-16 ms) aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC (CT-27 ms) aliphaticsample_1isotropicsample_conditions_1
3D C(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D-HCCH-COSY-alisample_1isotropicsample_conditions_1
3D HCCH-COSY-arosample_1isotropicsample_conditions_1
2D 1H-13C HSQC (CT-56 ms) aliphaticsample_2isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis,refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - data analysis,chemical shift assignment

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment

VNMRJ, Varian - collection

CSI, (CSI) Wishart and Sykes - Secondary structure analysis

PROSA v6.4, Guntert - processing

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

PSVS, Bhattacharya, Montelione - structure validation

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

UNP O75147
NCBI 23363
PDB