BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18490

Title: TDRD3 complex   PubMed: 23066109

Authors: Sikorsky, Tomas

Citation: Sikorsky, Tomas; Hobor, Fruzsina; Krizanova, Eva; Pasulka, Josef; Kubicek, Karel; Stefl, Richard. "Recognition of asymmetrically dimethylated arginine by TDRD3."  Nucleic Acids Res. 40, 11748-11755 (2012).

Assembly members:
entity_1, polymer, 58 residues, 6827.864 Da.
TDRD3_2, polymer, 13 residues, 1494.620 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MKMWKPGDECFALYWEDNKF YRAEVEALHSSGMTAVVKFI DYGNYEEVLLSNIKPILE
TDRD3_2: YSPSSPXYTPQSP

Data sets:
Data typeCount
13C chemical shifts176
15N chemical shifts53
1H chemical shifts346

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TDRD3, chain 11
2TDRD3, chain 22

Entities:

Entity 1, TDRD3, chain 1 58 residues - 6827.864 Da.

1   METLYSMETTRPLYSPROGLYASPGLUCYS
2   PHEALALEUTYRTRPGLUASPASNLYSPHE
3   TYRARGALAGLUVALGLUALALEUHISSER
4   SERGLYMETTHRALAVALVALLYSPHEILE
5   ASPTYRGLYASNTYRGLUGLUVALLEULEU
6   SERASNILELYSPROILELEUGLU

Entity 2, TDRD3, chain 2 13 residues - 1494.620 Da.

1   TYRSERPROSERSERPRODA2TYRTHRPRO
2   GLNSERPRO

Samples:

sample_1: TDRD3_1, [U-99% 13C; U-99% 15N], 1.7 mM; sodium phosphate 50 mM; beta-mercaptoethanol 10 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 8; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB14950 BAJ17879 BAK63800
EMBL CAD97894 CAL37786
GB AAH30514 AAH60876 ACC94142 AIC52422 EAW52079
REF NP_001139542 NP_001139543 NP_110421 XP_003257452 XP_003257453
SP Q9H7E2