BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18477

Title: NMR dynamics in the C-terminal globular domain of oligosaccharyltransferase   PubMed: 23177926

Authors: Nyirenda, James; Matsumoto, Shunsuke; Takashi, Saitoh; Kohda, Daisuke

Citation: Nyirenda, James; Matsumoto, Shunsuke; Saitoh, Takashi; Maita, Nobuo; Noda, Nobuo; Inagaki, Fuyuhiko; Kohda, Daisuke. "Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance."  Structure 21, 32-41 (2013).

Assembly members:
AfAglB-S2, polymer, 180 residues, Formula weight is not available

Natural source:   Common Name: euryarchaeotes   Taxonomy ID: 2234   Superkingdom: Archae   Kingdom: not available   Genus/species: Archaeoglobus fulgidus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
AfAglB-S2: MAHHHHHHSAALEVLFQGPE MTMDWKEALNWMKENLEAQD YLKAYEKPDYAVLSWWDYGN WILYVAKKAVVCNNFQAGAD DAAKFFTAQSEEEAMKIVEK RKVRYVVTVEELTVKPETNK TKFIPIMQIAGYSPEYMKNK EIIDFFNKTMLYKLHVENAT NLTHFRLLKNFGTVKIFEVK

Data typeCount
15N chemical shifts151
1H chemical shifts151
heteronuclear NOE values264
order parameters130
T1 relaxation values264
T2 relaxation values264

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AfAglB-S21

Entities:

Entity 1, AfAglB-S2 180 residues - Formula weight is not available

Residues 1-19 are plasmid derived and part of the sequence in the assigned residues is labeled 10-18 from Alanine number 10 and the target sequence starts from 433.

1   METALAHISHISHISHISHISHISSERALA
2   ALALEUGLUVALLEUPHEGLNGLYPROGLU
3   METTHRMETASPTRPLYSGLUALALEUASN
4   TRPMETLYSGLUASNLEUGLUALAGLNASP
5   TYRLEULYSALATYRGLULYSPROASPTYR
6   ALAVALLEUSERTRPTRPASPTYRGLYASN
7   TRPILELEUTYRVALALALYSLYSALAVAL
8   VALCYSASNASNPHEGLNALAGLYALAASP
9   ASPALAALALYSPHEPHETHRALAGLNSER
10   GLUGLUGLUALAMETLYSILEVALGLULYS
11   ARGLYSVALARGTYRVALVALTHRVALGLU
12   GLULEUTHRVALLYSPROGLUTHRASNLYS
13   THRLYSPHEILEPROILEMETGLNILEALA
14   GLYTYRSERPROGLUTYRMETLYSASNLYS
15   GLUILEILEASPPHEPHEASNLYSTHRMET
16   LEUTYRLYSLEUHISVALGLUASNALATHR
17   ASNLEUTHRHISPHEARGLEULEULYSASN
18   PHEGLYTHRVALLYSILEPHEGLUVALLYS

Samples:

sample_1: AfAglB-S2, [U-99% 13C; U-99% 15N], 1.0 mM; AfAglB-S2, [U-99% 15N], 1.0 mM; H2O 90%; D2O 10%

Sample_2: AfAglB-S2, [U-99% 15N], 0.5-1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 5 mM; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
1H-15N heteronuclear noeSample_2isotropicsample_conditions_2
1H-15N heteronuclear noeSample_2isotropicsample_conditions_2
2D 1H-15N HSQCSample_2isotropicsample_conditions_2
2D 1H-15N HSQCSample_2isotropicsample_conditions_2

Software:

TOPSPIN v2, Bruker Biospin - collection

NMRPipe, Delaglio, Zhengrong and Bax - processing

SPARKY, Goddard - chemical shift assignment

ModelFree v4.16, Palmer - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

GB AAB91198
REF WP_048064157