BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18468

Title: Solution NMR structure of the specialized acyl carrier protein PA3334 (apo) from Pseudomonas aeruginosa. Northeast Structural Genomics Consortium Target PaT415.

Authors: Ramelot, Theresa; Cort, John; Yang, Yunhuang; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michael

Citation: Ramelot, Theresa; Cort, John; Yang, Yunhuang; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michael. "Solution NMR structure of the specialized acyl carrier protein PA3334 (apo) from Pseudomonas aeruginosa. Northeast Structural Genomics Consortium Target PaT415."  Not known ., .-..

Assembly members:
PaT415, polymer, 103 residues, 12.2 Da.

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 297   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PaT415: MGSSHDHHHHSSGRENLYFQ GHMPNDMEDHLLTVLSVASG VPKEEISRDSRMEDLAFDSL VVSELSLKLRKEFGVTGVDD ELDLLETVDELFQLVEKHRA AGS

Data sets:
Data typeCount
13C chemical shifts370
15N chemical shifts76
1H chemical shifts596

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PaT4151

Entities:

Entity 1, PaT415 103 residues - 12.2 Da.

1   METGLYSERSERHISASPHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETPROASNASPMETGLUASPHIS
4   LEULEUTHRVALLEUSERVALALASERGLY
5   VALPROLYSGLUGLUILESERARGASPSER
6   ARGMETGLUASPLEUALAPHEASPSERLEU
7   VALVALSERGLULEUSERLEULYSLEUARG
8   LYSGLUPHEGLYVALTHRGLYVALASPASP
9   GLULEUASPLEULEUGLUTHRVALASPGLU
10   LEUPHEGLNLEUVALGLULYSHISARGALA
11   ALAGLYSER

Samples:

sample_1: PaT415, [U-100% 13C; U-100% 15N], 1.0 mM; TRIS 10 mM; sodium chloride 450 mM; DTT 10 mM; zinc chloride 0.01 mM; sodium azide 0.01%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 250 mM; pH: 7.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
4D 1H-13C-13C-1H NOESY in D2Osample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSY in D2Osample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

FMCGUI v2.5 linux, (FMCGUI) Alex Lemak - refinement

SPARKY v3.113, Goddard - peak picking

TALOS+ vVersion 1.2009.0721.18, Shen, Cornilescu, Delaglio and Bax - predict dihedral angles

MDDGUI vJune2009, Alex Lemak, University of Toronto - processing

NMRPipe vNMRPipe-2008, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AvanceIII 850 MHz
  • Bruker AvanceIII 600 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

UNP A3LDD1
BMRB 18819
PDB
DBJ BAK91288 BAP22043 BAP49673 BAQ38558 BAR66624
EMBL CAW26458 CCQ85541 CDH69992 CDH76078 CDI91738
GB AAG06722 ABJ12585 AEO74118 AFM63813 AGI80541
REF NP_252024 WP_003091713 WP_003106204 WP_034068812 WP_039700511