BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18406

Title: Solid-state NMR sequential assignments of Sup35NM   PubMed: 23934139

Authors: Schuetz, Anne; Luckgei, Nina; Habenstein, Birgit; Meier, Beat; Boeckmann, Anja

Citation: Luckgei, Nina; Schuetz, Anne; Habenstein, Birgit; Bousset, Luc; Sourigues, Yannick; Melki, Ronald; Meier, Beat; Boeckmann, Anja. "Solid-state NMR sequential assignments of the amyloid core of Sup35pNM"  Biomol. NMR Assign. ., .-. (2013).

Assembly members:
Sup35NM, polymer, 273 residues, 30685.8032 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sup35NM: MGSSHHHHHHSSGLVPRGSH MSDSNQGNNQQNYQQYSQNG NQQQGNNRYQGYQAYNAQAQ PAGGYYQNYQGYSGYQQGGY QQYNPDAGYQQQYNPQGGYQ QYNPQGGYQQQFNPQGGRGN YKNFNYNNNLQGYQAGFQPQ SQGMSLNDFQKQQKQAAPKP KKTLKLVSSSGIKLANATKK VGTKPAESDKKEEEKSAETK EPTKEPTKVEEPVKKEEKPV QTEEKTEEKSELPKVEDLKI SESTHNTNNANVTSADALIK EQEEEVDDEVVND

Data sets:
Data typeCount
13C chemical shifts85
15N chemical shifts31

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sup35NM1

Entities:

Entity 1, Sup35NM 273 residues - 30685.8032 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASPSERASNGLNGLYASNASNGLN
4   GLNASNTYRGLNGLNTYRSERGLNASNGLY
5   ASNGLNGLNGLNGLYASNASNARGTYRGLN
6   GLYTYRGLNALATYRASNALAGLNALAGLN
7   PROALAGLYGLYTYRTYRGLNASNTYRGLN
8   GLYTYRSERGLYTYRGLNGLNGLYGLYTYR
9   GLNGLNTYRASNPROASPALAGLYTYRGLN
10   GLNGLNTYRASNPROGLNGLYGLYTYRGLN
11   GLNTYRASNPROGLNGLYGLYTYRGLNGLN
12   GLNPHEASNPROGLNGLYGLYARGGLYASN
13   TYRLYSASNPHEASNTYRASNASNASNLEU
14   GLNGLYTYRGLNALAGLYPHEGLNPROGLN
15   SERGLNGLYMETSERLEUASNASPPHEGLN
16   LYSGLNGLNLYSGLNALAALAPROLYSPRO
17   LYSLYSTHRLEULYSLEUVALSERSERSER
18   GLYILELYSLEUALAASNALATHRLYSLYS
19   VALGLYTHRLYSPROALAGLUSERASPLYS
20   LYSGLUGLUGLULYSSERALAGLUTHRLYS
21   GLUPROTHRLYSGLUPROTHRLYSVALGLU
22   GLUPROVALLYSLYSGLUGLULYSPROVAL
23   GLNTHRGLUGLULYSTHRGLUGLULYSSER
24   GLULEUPROLYSVALGLUASPLEULYSILE
25   SERGLUSERTHRHISASNTHRASNASNALA
26   ASNVALTHRSERALAASPALALEUILELYS
27   GLUGLNGLUGLUGLUVALASPASPGLUVAL
28   VALASNASP

Samples:

Sup35NM_1: Sup35NM, [U-100% 13C; U-100% 15N], 15 ± 2 mg

Condition: ionic strength: 0 M; pH: 7.000; pressure: 1.000 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
DARR 20 msSup35NM_1solidCondition
NCASup35NM_1solidCondition
NCACBSup35NM_1solidCondition
CANCOSup35NM_1solidCondition
NCOCXSup35NM_1solidCondition
NCACOSup35NM_1solidCondition
DARR 100 msSup35NM_1solidCondition
NCOCASup35NM_1solidCondition
NCACXSup35NM_1solidCondition
CCCSup35NM_1solidCondition
HC INEPTSup35NM_1solidCondition
NCOSup35NM_1solidCondition

Software:

ANALYSIS v2.1, CCPN - chemical shift assignment

CcpNMR vany, CCPN - Spectrum analysis, Spectrum display

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

BMRB 15379 18407
DBJ GAA22402
EMBL CAA30155 CAA68760 CAA86677 CAY78675
GB AAA35133 AAF99684 AAK26176 AAK26180 AAK26182
REF NP_010457
SP P05453
TPG DAA12014