BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18399

Title: Solution structure of a ubiquitin-like protein from Trypanosoma brucei

Authors: Wang, Rui; Wang, Tao; Liao, Shanhui; Zhang, Jiahai; Tu, Xiaoming

Citation: Wang, Rui; Liao, Shanhui; Wang, Tao; Zhang, Jiahai; Tu, Xiaoming. "Solution structure of a ubiqutin-like protein from Trypanosoma bucei"  Not known ., .-..

Assembly members:
entity, polymer, 84 residues, 8680.189 Da.

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: HHHHHHMLLKVKTVSNKVIQ ITSLTDDNTIAELKGKLEES EGIPGNMIRLVYQGKQLEDE KRLKDYQMSAGATFHMVVAL RAGC

Data sets:
Data typeCount
13C chemical shifts224
15N chemical shifts77
1H chemical shifts447

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiqutin-like protein from Trypanosoma bucei1

Entities:

Entity 1, ubiqutin-like protein from Trypanosoma bucei 84 residues - 8680.189 Da.

1   HISHISHISHISHISHISMETLEULEULYS
2   VALLYSTHRVALSERASNLYSVALILEGLN
3   ILETHRSERLEUTHRASPASPASNTHRILE
4   ALAGLULEULYSGLYLYSLEUGLUGLUSER
5   GLUGLYILEPROGLYASNMETILEARGLEU
6   VALTYRGLNGLYLYSGLNLEUGLUASPGLU
7   LYSARGLEULYSASPTYRGLNMETSERALA
8   GLYALATHRPHEHISMETVALVALALALEU
9   ARGALAGLYCYS

Samples:

sample_1: tbubl, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 0.5 mM; D2O 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift calculation

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
EMBL CBH10548
GB AAX79072 AAZ10845
REF XP_011772837 XP_844404