BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18397

Title: nanocrystalline GB1 1H, 13C, 15N assignments   PubMed: 22986689

Authors: Zhou, Donghua

Citation: Zhou, Donghua; Nieuwkoop, Andrew; Berthold, Deborah; Comellas, Gemma; Sperling, Lindsay; Tang, Ming; Shah, Gautam; Brea, Elliott; Lemkau, Luisel; Rienstra, Chad. "Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy."  J. Biomol. NMR 54, 291-305 (2012).

Assembly members:
GB1, polymer, 56 residues, Formula weight is not available

Natural source:   Common Name: Streptococcal bacteria   Taxonomy ID: 1301   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcal bacteria

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GB1: MQYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTE

Data sets:
Data typeCount
13C chemical shifts186
15N chemical shifts61
1H chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB11

Entities:

Entity 1, GB1 56 residues - Formula weight is not available

1   METGLNTYRLYSLEUILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

sample_1: GB1, [U-98% 13C; U-98% 15N], 5 mg

sample_conditions_1: pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
NHsample_1solidsample_conditions_1
CANHsample_1solidsample_conditions_1
CA(CO)NHsample_1solidsample_conditions_1
CANHsample_1solidsample_conditions_1
CONHsample_1solidsample_conditions_1
CO(CA)NHsample_1solidsample_conditions_1
CBCANHsample_1solidsample_conditions_1
CBCA(CO)NHsample_1solidsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 15156 15380 16444 16627 16755 16873 16882 16958 17810 19394 26630
PDB
EMBL CAA37410
GB AAY41168