BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18351

Title: Solution structure of the Class II hydrophobin NC2   PubMed: 24218020

Authors: Ren, Qin; Kwan, Ann; Sunde, Margaret

Citation: Ren, Qin; Kwan, Ann; Sunde, Margaret. "Solution structure and interface-driven self-assembly of NC2, a new member of the Class II hydrophobin proteins."  Proteins ., .-. (2013).

Assembly members:
nc2, polymer, 80 residues, 8187.3024 Da.

Natural source:   Common Name: Neurospora crassa   Taxonomy ID: 5141   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Neurospora crassa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
nc2: SPAAMERQVPYTPCSGLYGT AQCCATDVLGVADLDCANPP ATLANATHFESTCAAIGQRA RCCVLPILGQDILCQTPAGL

Data typeCount
13C chemical shifts327
15N chemical shifts88
1H chemical shifts536
T1 relaxation values71
T2 relaxation values71
heteronuclear NOE values71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nc21

Entities:

Entity 1, nc2 80 residues - 8187.3024 Da.

1   SERPROALAALAMETGLUARGGLNVALPRO
2   TYRTHRPROCYSSERGLYLEUTYRGLYTHR
3   ALAGLNCYSCYSALATHRASPVALLEUGLY
4   VALALAASPLEUASPCYSALAASNPROPRO
5   ALATHRLEUALAASNALATHRHISPHEGLU
6   SERTHRCYSALAALAILEGLYGLNARGALA
7   ARGCYSCYSVALLEUPROILELEUGLYGLN
8   ASPILELEUCYSGLNTHRPROALAGLYLEU

Samples:

unlabeled: nc2 0.3 mM; DSS 0.3 mM; Sodium phosphate 20.0 mM

13C15N_01: nc2, [U-100% 13C; U-100% 15N], 0.35 mM; Sodium phosphate 20.00 mM; DSS 0.30 mM

sample_new_1: Sodium phosphate 20 mM; nc2, [U-13C; U-15N], 0.3 mM

13C15N_03: nc2, [U-100% 13C; U-100% 15N], 0.3 mM; Sodium phosphate 20.0 mM; DSS 0.3 mM

13C15N_02: nc2, [U-100% 13C; U-100% 15N], 0.3 mM; Sodium phosphate 20.0 mM; DSS 0.3 mM

15N: nc2, [U-100% 15N], 0.3 mM; DSS 0.3 mM; Sodium phosphate 20.0 mM

T2-1: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-2: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-3: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-4: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-5: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-6: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-7: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-8: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-9: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-10: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-11: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-12: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-13: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-14: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T2-15: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-1: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-2: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-3: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-4: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-5: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-6: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-7: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-8: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-9: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-10: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-11: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

T1-12: ionic strength: 20.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

CondSet1: ionic strength: 20.000 mM; pH: 6.000; temperature: 283.000 K

Experiments:

NameSampleSample stateSample conditions
T2:1 (H[n[T2(N)]])13C15N_03isotropicT2-1
T2:2 (H[n[T2(N)]])13C15N_03isotropicT2-2
T2:3 (H[n[T2(N)]])13C15N_03isotropicT2-3
T2:4 (H[n[T2(N)]])13C15N_03isotropicT2-4
T2:5 (H[n[T2(N)]])13C15N_03isotropicT2-5
T2:6 (H[n[T2(N)]])13C15N_03isotropicT2-6
T2:7 (H[n[T2(N)]])13C15N_03isotropicT2-7
T2:8 (H[n[T2(N)]])13C15N_03isotropicT2-8
T2:9 (H[n[T2(N)]])13C15N_03isotropicT2-9
T2:10 (H[n[T2(N)]])13C15N_03isotropicT2-10
T2:11 (H[n[T2(N)]])13C15N_03isotropicT2-11
T2:12 (H[n[T2(N)]])13C15N_03isotropicT2-12
T2:13 (H[n[T2(N)]])13C15N_03isotropicT2-13
T2:14 (H[n[T2(N)]])13C15N_03isotropicT2-14
T2:15 (H[n[T2(N)]])13C15N_03isotropicT2-15
T1:1 (H[n[T1(N)]])13C15N_03isotropicT1-1
T1:2 (H[n[T1(N)]])13C15N_03isotropicT1-2
T1:3 (H[n[T1(N)]])13C15N_03isotropicT1-3
T1:4 (H[n[T1(N)]])13C15N_03isotropicT1-4
T1:5 (H[n[T1(N)]])13C15N_03isotropicT1-5
T1:6 (H[n[T1(N)]])13C15N_03isotropicT1-6
T1:7 (H[n[T1(N)]])13C15N_03isotropicT1-7
T1:8 (H[n[T1(N)]])13C15N_03isotropicT1-8
T1:9 (H[n[T1(N)]])13C15N_03isotropicT1-9
T1:10 (H[n[T1(N)]])13C15N_03isotropicT1-10
T1:11 (H[n[T1(N)]])13C15N_03isotropicT1-11
T1:12 (H[n[T1(N)]])13C15N_03isotropicT1-12
htNOE 1 (H[n[Trho(N)]])13C15N_03isotropicCondSet1
htNOE 2 (H[n[Trho(N)]])13C15N_03isotropicCondSet1
3D 1H-13C NOESY13C15N_02isotropicCondSet1
3D 1H-13C NOESY13C15N_02isotropicCondSet1
3D 1H-15N NOESY15NisotropicCondSet1
2D 1H-1H NOESYunlabeledisotropicCondSet1
3D HNCO13C15N_01isotropicCondSet1
HNcaCO (H[N[ca[CO]]])13C15N_01isotropicCondSet1
T2sample_new_1solutionCondSet1
T1sample_new_1solutionCondSet1
HTNOE 1sample_new_1solutionCondSet1

Software:

ARIA v2.3, BENJAMIN BARDIAUX, MICHAEL HABECK, JENS - solution structure and refinement

AutoDep v4.3, PDBe - collection

ANALYSIS v2.1, CCPN - peak picking and data analysis

DANGLE v1.1, Cheung, Maguire, Stevens and Broadhurst - Secondary structure prediction

MOLMOL_2K.2 vany, Koradi, Billeter and Wuthrich - chemical shift assignment

Molmol v2K.2, Koradi, Billeter and Wuthrich - data analysis

MolProbity v3.19, Ian W. Davis, Vincent B. Chen, Robert M. Immormino, Jeff J. Headd, W. Bryan Arendall, J. Michael Word, David C. Richardson - Validation

ProcheckNMR v3.4, Laskowski and MacArthur - structure validation

TOPSPIN v2.1, Bruker Biospin - data collection

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker AVANCE 800 MHz

Related Database Links:

GenBank TmpAcc
UNP Q7S3P5_NEUCR
PDB
GB EAA30046 KHE86439
REF XP_959282