BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18342

Title: Backbone and sidechain assignments for Get5_UBL domain   PubMed: 23001946

Authors: Simon, Aline; Simpson, Peter; Isaacson, Rivka

Citation: Simon, Aline; Simpson, Peter; Hawthorne, William; Hale, Lisa; Goldstone, Rachael; Isaacson, Rivka. "1H, 13C and 15N assignments of Sgt2N-terminal dimerisation domain and its binding partner, Get5 Ubiquitin-like domain."  Biomol. NMR Assignments 7, 271-274 (2013).

Assembly members:
Get5_ubl, polymer, 97 residues, 10963.5 Da.

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Get5_ubl: AHHHHHHVDDDDKMDNAAVH LTLKKIQAPKFSIEHDFSPS DTILQIKQHLISEEKASHIS EIKLLLKGKVLHDNLFLSDL KVTPANSTITVMIKPNP

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts82
1H chemical shifts601

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Get5_ubl1

Entities:

Entity 1, Get5_ubl 97 residues - 10963.5 Da.

1   ALAHISHISHISHISHISHISVALASPASP
2   ASPASPLYSMETASPASNALAALAVALHIS
3   LEUTHRLEULYSLYSILEGLNALAPROLYS
4   PHESERILEGLUHISASPPHESERPROSER
5   ASPTHRILELEUGLNILELYSGLNHISLEU
6   ILESERGLUGLULYSALASERHISILESER
7   GLUILELYSLEULEULEULYSGLYLYSVAL
8   LEUHISASPASNLEUPHELEUSERASPLEU
9   LYSVALTHRPROALAASNSERTHRILETHR
10   VALMETILELYSPROASNPRO

Samples:

sample_1: Get5_ubl, [U-98% 13C; U-98% 15N], 700 uM; MES 100 mM; potassium chloride 150 mM; TCEP 0.5 mM; H2O 90%; D2O 10%

sample_2: Get5_ubl, [U-98% 13C; U-98% 15N], 700 uM; MES 100 mM; potassium chloride 150 mM; TCEP 0.5 mM; D2O 100%

sample_conditions_1: ionic strength: 0.25 M; pH: 6; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18669 18671
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