BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18300

Title: Solution NMR structure of asteropusin A from marine sponge Asteropus sp.   PubMed: 23201194

Authors: Li, Huayue; Bowling, John; Hamann, Mark; Jung, Jee

Citation: Li, Huayue; Bowling, John; Fronczek, Frank; Hong, Jongki; Jabbae, Sairam; Murray, Thomas; Haa, Nam-Chul; Hamann, Mark; Jung, Jee. "A New Knottin from a Marine Sponge Which Enhances Neuronal Ca2+ Influx"  Biochim. Biophys. Acta 1830, 2591-2599 (2012).

Assembly members:
Asteropusin_A, polymer, 37 residues, 3904.393 Da.

Natural source:   Common Name: sponges   Taxonomy ID: 350938   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Asteropus sp.

Experimental source:   Production method: purified from the natural source   Host organism: Asteropus sp.

Entity Sequences (FASTA):
Asteropusin_A: XGCAFEGESCNVQFYPCCPG LGLTCIPGNPDGTCYYL

Data sets:
Data typeCount
13C chemical shifts118
1H chemical shifts228

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Asteropusin_A1

Entities:

Entity 1, Asteropusin_A 37 residues - 3904.393 Da.

1   PCAGLYCYSALAPHEGLUGLYGLUSERCYS
2   ASNVALGLNPHETYRPROCYSCYSPROGLY
3   LEUGLYLEUTHRCYSILEPROGLYASNPRO
4   ASPGLYTHRCYSTYRTYRLEU

Samples:

ABU8-1: methanol 10 mM

ABU8-1_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYABU8-1isotropicABU8-1_conditions_1
2D 1H-1H TOCSYABU8-1isotropicABU8-1_conditions_1
2D DQF-COSYABU8-1isotropicABU8-1_conditions_1
2D 1H-13C HSQC aliphaticABU8-1isotropicABU8-1_conditions_1
2D 1H-13C HSQC aromaticABU8-1isotropicABU8-1_conditions_1

Software:

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 18783
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