BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18292

Title: NMR structure of the RNA binding motif 39 (RBM39) from Mus musculus

Authors: Serrano, Pedro; Dutta, Samit; Geralt, Michael; Wuthrich, Kurt

Citation: Serrano, Pedro; Dutta, Samit; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the RNA binding motif 39 (RBM39) from Mus musculus"  Not known ., .-..

Assembly members:
entity, polymer, 113 residues, 12516.306 Da.

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: VQPLATQCFQLSNMFNPQTE EEVGWDTEIKDDVIEECNKH GGVIHIYVDKNSAQGNVYVK CPSIAAAIAAVNALHGRWFA GKMITAAYVPLPTYHNLFPD SMTATQLLVPSRR

Data sets:
Data typeCount
13C chemical shifts476
15N chemical shifts120
1H chemical shifts756

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBM391

Entities:

Entity 1, RBM39 113 residues - 12516.306 Da.

1   VALGLNPROLEUALATHRGLNCYSPHEGLN
2   LEUSERASNMETPHEASNPROGLNTHRGLU
3   GLUGLUVALGLYTRPASPTHRGLUILELYS
4   ASPASPVALILEGLUGLUCYSASNLYSHIS
5   GLYGLYVALILEHISILETYRVALASPLYS
6   ASNSERALAGLNGLYASNVALTYRVALLYS
7   CYSPROSERILEALAALAALAILEALAALA
8   VALASNALALEUHISGLYARGTRPPHEALA
9   GLYLYSMETILETHRALAALATYRVALPRO
10   LEUPROTHRTYRHISASNLEUPHEPROASP
11   SERMETTHRALATHRGLNLEULEUVALPRO
12   SERARGARG

Samples:

sample_1: RBM39, [U-95% 13C; U-95% 15N], 1.2 mM; sodium azide 4.5 mM; sodium chloride 50 mM; sodium phosphate 25 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.083 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
4D APSY HACANHsample_1isotropicsample_conditions_1
5D APSY CBCACONHsample_1isotropicsample_conditions_1
5D APSY HACACONHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

Opalp, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - collection, data analysis, processing

UNIO, Unio Herrmann Wuthrich - chemical shift assignment, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAE22009 BAE22477 BAE27657 BAE32977 BAG54289
EMBL CAD97833 CAE45833 CAE45890 CAH18281 CAH90627
GB AAA16346 AAA16347 AAH04000 AAH30493 AAH82607
REF NP_001013225 NP_001125339 NP_001162566 NP_001164806 NP_001193433
SP Q14498 Q5RC80 Q8VH51
TPG DAA23046