BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18232

Title: Solid-state NMR assignments for mouse alpha-synuclein fibrils   PubMed: 22516611

Authors: Lv, Guohua; Kumar, Ashutosh; Giller, Karin; Orcellet, Maria; Riedel, Dietmar; Fernandez, Claudio; Becker, Stefan

Citation: Lv, Guohua; Kumar, Ashutosh; Giller, Karin; Orcellet, Maria; Riedel, Dietmar; Fernandez, Claudio; Becker, Stefan; Lange, Adam. "Structural Comparison of Mouse and Human -Synuclein Amyloid Fibrils by Solid-State NMR."  J. Mol. Biol. 420, 99-111 (2012).

Assembly members:
mouse_alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mouse_alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVTTVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGNIAAATGFVKKDQM GKGEEGYPQEGILEDMPVDP GSEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts213
15N chemical shifts60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse alpha-synuclein1

Entities:

Entity 1, mouse alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALTHRTHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYASNILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNMET
11   GLYLYSGLYGLUGLUGLYTYRPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   GLYSERGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: mouse alpha-synuclein, [U-100% 13C; U-100% 15N], 10 mg; TRIS 25 mM

sample_2: mouse alpha-synuclein, [2-Glucose 13C; U-100% 15N], 10 mg; TRIS 25 mM; DSS 0.1-0.5 mg

sample_3: mouse alpha-synuclein, [1-Glucose 13C; U-100% 15N], 10 mg; TRIS 25 mM; DSS 0.1-0.5 mg

sample_4: mouse alpha-synuclein, mixed 1:1 13C:15N, 10 mg; TRIS 25 mM; DSS 0.1-0.5 mg

sample_conditions_1: pH: 7.5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D PDSD 20 mssample_1solidsample_conditions_1
2D PDSD 150 mssample_1solidsample_conditions_1
2D DREAMsample_1solidsample_conditions_1
2D NCACX PDSDsample_1solidsample_conditions_1
2D NCOCX PDSDsample_1solidsample_conditions_1
3D NCACX PDSDsample_1solidsample_conditions_1
3D NCOCX PDSDsample_1solidsample_conditions_1
2D NCAsample_1solidsample_conditions_1
2D NHHCsample_1solidsample_conditions_1
2D PDSD 500 mssample_2solidsample_conditions_1
2D PDSD 700 mssample_2solidsample_conditions_1
2D NCAsample_2solidsample_conditions_1
2D NCOsample_2solidsample_conditions_1
2D NHHCsample_2solidsample_conditions_1
2D PDSD 400 mssample_3solidsample_conditions_1
2D NCAsample_3solidsample_conditions_1
2D NCOsample_3solidsample_conditions_1
2D NHHCsample_4solidsample_conditions_1
2D DQ-SQ SPC5sample_1solidsample_conditions_1
2D NCACX DARRsample_1solidsample_conditions_1
2D NCOCX DARRsample_1solidsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 19346 19347 19348 19349
DBJ BAB29375 BAE33670
GB AAB20688 AAC00521 AAC16026 AAD11254 AAD56907
REF NP_001035916 NP_033247 NP_062042 XP_003503776 XP_004648456
SP O55042 P37377