BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18208

Title: Chemical shift assignments of the E46K alpha-synuclein fibrils   PubMed: 23505409

Authors: Lemkau, Luisel; Comellas, Gemma; Lee, Shin; Rikardsen, Lars; Woods, Wendy; George, Julia; Rienstra, Chad

Citation: Lemkau, Luisel; Comellas, Gemma; Lee, Shin; Rikardsen, Lars; Woods, Wendy; George, Julia; Rienstra, Chad. "Site-Specific Perturbations of Alpha-Synuclein Fibril Structure by the Parkinson's Disease Associated Mutations A53T and E46K."  PLoS ONE 8, e49750-e49750 (2013).

Assembly members:
E46K_alpha-synuclein_fibrils, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
E46K_alpha-synuclein_fibrils: MDVFMKGKSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKKGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts133
15N chemical shifts38

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E46K AS1

Entities:

Entity 1, E46K AS 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLYSSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSLYSGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: E46K alpha-synuclein fibrils, [U-13C; U-15N], 35%; H2O, natural source, 35%

sample_conditions_1: pH: 7.4; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CAN(co)CXsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian UnityPlus 600 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17665 17910 18207 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001129014 NP_001139526 NP_001139527
SP P37840 P61139 P61140 P61142 P61143