BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18182

Title: Identification and structural basis for a novel interaction between Vav2 and Arap3   PubMed: 22750419

Authors: Wu, Bo; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu

Citation: Wu, Bo; Wang, Fengsong; Zhang, Jiahai; Zhang, Zhiyong; Qin, Liying; Peng, Junhui; Li, Fudong; Liu, Jianping; Lu, Guowei; Gong, Qingguo; Yao, Xuebiao; Wu, Jihui; Shi, Yunyu. "Identification and structural basis for a novel interaction between Vav2 and Arap3"  J. Struct. Biol. 180, 84-95 (2012).

Assembly members:
entity_1, polymer, 113 residues, 13480.319 Da.
entity_2, polymer, 9 residues, 1130.064 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: SRPPSREIDYTAYPWFAGNM ERQQTDNLLKSHASGTYLIR ERPAEAERFAISIKFNDEVK HIKVVEKDNWIHITEAKKFD SLLELVEYYQCHSLKESFKQ LDTTLKYPYKSRE
entity_2: EEPVXEEVG

Data sets:
Data typeCount
13C chemical shifts455
15N chemical shifts111
1H chemical shifts884

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vav21
2Arap32

Entities:

Entity 1, Vav2 113 residues - 13480.319 Da.

1   SERARGPROPROSERARGGLUILEASPTYR
2   THRALATYRPROTRPPHEALAGLYASNMET
3   GLUARGGLNGLNTHRASPASNLEULEULYS
4   SERHISALASERGLYTHRTYRLEUILEARG
5   GLUARGPROALAGLUALAGLUARGPHEALA
6   ILESERILELYSPHEASNASPGLUVALLYS
7   HISILELYSVALVALGLULYSASPASNTRP
8   ILEHISILETHRGLUALALYSLYSPHEASP
9   SERLEULEUGLULEUVALGLUTYRTYRGLN
10   CYSHISSERLEULYSGLUSERPHELYSGLN
11   LEUASPTHRTHRLEULYSTYRPROTYRLYS
12   SERARGGLU

Entity 2, Arap3 9 residues - 1130.064 Da.

1   GLUGLUPROVALPTRGLUGLUVALGLY

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 1 mM; entity_2 3 mM; potassium phosphate 5 mM; sodium phosphate 20 mM; DTT 5 mM; EDTA 2 mM; sodium chloride 75 mM; H2O 90%; D2O 10%

sample_2: entity_1, [U-100% 13C; U-100% 15N], 1 mM; entity_2 3 mM; potassium phosphate 5 mM; sodium chloride 75 mM; DTT 5 mM; EDTA 2 mM; sodium phosphate 20 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 100 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
3D HCCH-COSYsample_2isotropicsample_conditions_2
3D 1H-13C NOESYsample_2isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift calculation

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 18183
PDB
DBJ BAJ17841
EMBL CAE45861 CAH89854
GB AAB34377 AAH33187 AAI32966 AAI32968 EAW88108
REF NP_001124861 NP_001127870 NP_001252562 NP_003362 XP_003911174
SP P52735