BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18171

Title: Solution structure of atTic-hip/hop domain (Residue 310-371)   PubMed: 22888115

Authors: Chen, Chinpan; Kao, Yi-Fen

Citation: Kao, Yi-Fen; Lou, Yuan-Chao; Yeh, Yi-Hung; Hsiao, Chwan-Deng; Chen, Chinpan. "Solution structure of the C-terminal NP-repeat domain of Tic40, a co-chaperone during protein import into chloroplasts."  J. Biochem. 152, 443-451 (2012).

Assembly members:
entity, polymer, 62 residues, 7056.178 Da.

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: PEEVISKIMENPDVAMAFQN PRVQAALMECSENPMNIMKY QNDKEVMDVFNKISQLFPGM TG

Data sets:
Data typeCount
13C chemical shifts262
15N chemical shifts67
1H chemical shifts425

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1atTic-hip/hop domain (Residue 310-371)1

Entities:

Entity 1, atTic-hip/hop domain (Residue 310-371) 62 residues - 7056.178 Da.

1   PROGLUGLUVALILESERLYSILEMETGLU
2   ASNPROASPVALALAMETALAPHEGLNASN
3   PROARGVALGLNALAALALEUMETGLUCYS
4   SERGLUASNPROMETASNILEMETLYSTYR
5   GLNASNASPLYSGLUVALMETASPVALPHE
6   ASNLYSILESERGLNLEUPHEPROGLYMET
7   THRGLY

Samples:

Tic40-Hip-Hop: potassium phosphate 20 mM; sodium citrate 20 mM; sodium chloride 100 mM; TFE, [U-99% 2H], 10%; H2O 90%; D2O 10%

Tic40-Hip-Hop_NMR_sample: ionic strength: 140 mM; pH: 5.56; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
2D 1H-13C HSQCTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D CBCA(CO)NHTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D HNCACBTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D HNCOTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D HN(CA)COTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D HNCATic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D HN(CO)CATic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D HCCH-TOCSYTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D CCCONHTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D 1H-15N NOESYTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D 1H-15N TOCSYTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
3D 1H-13C NOESYTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample
2D 1H-1H NOESYTic40-Hip-HopisotropicTic40-Hip-Hop_NMR_sample

Software:

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRView v5, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB10189
EMBL CDX69438 CDX85495 CDY38098
GB AAL16131 AAM13009 AAP31939 AED92318 EFH47986
REF NP_197165 XP_002871727 XP_006287761 XP_006287762 XP_006400200
SP Q9FMD5