BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18164

Title: Neurotensin 40 structures in DMPC:CHAPS:GM1 bicelle pH 5.5 298 K, NMR data & structures   PubMed: 22824320

Authors: Mukhopadhyay, Chaitali; Khatun, Ummul Liha

Citation: Khatun, Ummul; Goswami, Sudipto; Mukhopadhyay, Chaitali. "Modulation of the neurotensin solution structure in the presence of ganglioside GM1 bicelle"  Biophys. Chem. 168, 48-59 (2012).

Assembly members:
entity, polymer, 26 residues, 3369.949 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: ELYENKPRRPYILELYENKP RRPYIL

Data sets:
Data typeCount
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neurotensin1

Entities:

Entity 1, Neurotensin 26 residues - 3369.949 Da.

1   GLULEUTYRGLUASNLYSPROARGARGPRO
2   TYRILELEUGLULEUTYRGLUASNLYSPRO
3   ARGARGPROTYRILELEU

Samples:

sample_1: Neurotensin 4.5 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, NIH,T.D. Goddard, D.G. Kneller - chemical shift assignment, data analysis, refinement

SPARKY, NIH,T.D. Goddard, D.G. Kneller - chemical shift assignment, data analysis, refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 18162 18163