BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18154

Title: apo_YqcA   PubMed: 23749454

Authors: Jin, Changwen; Fu, Wenyu

Citation: Ye, Qian; Hu, Yunfei; Jin, Changwen. "(1)H, (13)C and (15)N resonance assignments of the apo and holo states of flavodoxin YqcA from Escherichia coli."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
entity, polymer, 149 residues, 16281.191 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MAEIGIFVGTMYGNSLLVAE EAEAILTAQGHKATVFEDPE LSDWLPYQDKYVLVVTSTTG QGDLPDSIVPLFQGIKDSLG FQPNLRYGVIALGDSSYVNF CNGGKQFDALLQEQSAQRVG EMLLIDASENPEPETESNPW VEQWGTLLS

Data sets:
Data typeCount
13C chemical shifts560
15N chemical shifts137
1H chemical shifts931

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YqcA from Escherichia coli1

Entities:

Entity 1, YqcA from Escherichia coli 149 residues - 16281.191 Da.

1   METALAGLUILEGLYILEPHEVALGLYTHR
2   METTYRGLYASNSERLEULEUVALALAGLU
3   GLUALAGLUALAILELEUTHRALAGLNGLY
4   HISLYSALATHRVALPHEGLUASPPROGLU
5   LEUSERASPTRPLEUPROTYRGLNASPLYS
6   TYRVALLEUVALVALTHRSERTHRTHRGLY
7   GLNGLYASPLEUPROASPSERILEVALPRO
8   LEUPHEGLNGLYILELYSASPSERLEUGLY
9   PHEGLNPROASNLEUARGTYRGLYVALILE
10   ALALEUGLYASPSERSERTYRVALASNPHE
11   CYSASNGLYGLYLYSGLNPHEASPALALEU
12   LEUGLNGLUGLNSERALAGLNARGVALGLY
13   GLUMETLEULEUILEASPALASERGLUASN
14   PROGLUPROGLUTHRGLUSERASNPROTRP
15   VALGLUGLNTRPGLYTHRLEULEUSER

Samples:

sample_1: protein, [U-15N], 1 mM; sodium phosphate 30 mM; DTT 40 mM; H2O 90%; D2O 10%

sample_2: protein, [U-13C; U-15N], 1 mM; sodium phosphate 30 mM; DTT 40 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 180 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 19151 19152 25013 25014
PDB
DBJ BAB37073 BAE76862 BAG78574 BAI27051 BAI32080
EMBL CAP77223 CAQ33114 CAQ99718 CAR04300 CAR09403
GB AAB40440 AAC75832 AAG57904 AAN44291 AAN81803
REF NP_311677 NP_417270 NP_708584 WP_000229279 WP_000807726
SP P65367 P65368 P65369