BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18135

Title: A single GalNAc residue on Threonine-106 modifies the dynamics and the structure of Interferon alpha-2a around the glycosylation site.   PubMed: 23184955

Authors: Ghasriani, Houman; Belcourt, Pascal; Sauve, Simon; Hodgson, Derek; Gingras, Genevieve; Brochu, Denis; Gilbert, Michel; Aubin, Yves

Citation: Ghasriani, Houman; Belcourt, Pascal; Sauve, Simon; Hodgson, Derek; Brochu, Denis; Gilbert, Michel; Aubin, Yves. "A single N-acetylgalactosamine residue at threonine 106 modifies the dynamics and structure of interferon 2a around the glycosylation site."  J. Biol. Chem. 288, 247-254 (2013).

Assembly members:
Interferon_Alpha-2a, polymer, 165 residues, 19264.242 Da.
A2G, non-polymer, 221.208 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Interferon_Alpha-2a: CDLPQTHSLGSRRTLMLLAQ MRKISLFSCLKDRHDFGFPQ EEFGNQFQKAETIPVLHEMI QQIFNLFSTKDSSAAWDETL LDKFYTELYQQLNDLEACVI QGVGVTETPLMKEDSILAVR KYFQRITLYLKEKKYSPCAW EVVRAEIMRSFSLSTNLQES LRSKE

Data sets:
Data typeCount
13C chemical shifts731
15N chemical shifts178
1H chemical shifts1197

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2SUGAR (N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE)2

Entities:

Entity 1, entity_1 165 residues - 19264.242 Da.

O-glycosylation at Thr106, with N-Acetyl-D-Galactosamine(alpha).

1   CYSASPLEUPROGLNTHRHISSERLEUGLY
2   SERARGARGTHRLEUMETLEULEUALAGLN
3   METARGLYSILESERLEUPHESERCYSLEU
4   LYSASPARGHISASPPHEGLYPHEPROGLN
5   GLUGLUPHEGLYASNGLNPHEGLNLYSALA
6   GLUTHRILEPROVALLEUHISGLUMETILE
7   GLNGLNILEPHEASNLEUPHESERTHRLYS
8   ASPSERSERALAALATRPASPGLUTHRLEU
9   LEUASPLYSPHETYRTHRGLULEUTYRGLN
10   GLNLEUASNASPLEUGLUALACYSVALILE
11   GLNGLYVALGLYVALTHRGLUTHRPROLEU
12   METLYSGLUASPSERILELEUALAVALARG
13   LYSTYRPHEGLNARGILETHRLEUTYRLEU
14   LYSGLULYSLYSTYRSERPROCYSALATRP
15   GLUVALVALARGALAGLUILEMETARGSER
16   PHESERLEUSERTHRASNLEUGLNGLUSER
17   LEUARGSERLYSGLU

Entity 2, SUGAR (N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE) - C8 H15 N O6 - 221.208 Da.

1   A2G

Samples:

sample_1: Interferon Alpha-2a (O-glycosylated), [U-100% 13C; U-100% 15N], 0.85 mM; Denaturated Sodium Acetate 25 mM; H2O 90%; D2O 10%

sample_2: Interferon Alpha-2a (O-glycosylated), [U-100% 13C; U-100% 15N], 0.53 mM; Deuturated Sodium Acetate 25 mM; H2O 90%; D2O 10%

sample_3: Interferon Alpha-2a (O-glycosylated), [U-100% 13C; U-100% 15N], 0.87 mM; Deuturated Sodium Acetate 25 mM; D2O 100%

sample_conditions_1: ionic strength: 25 mM; pH: 3.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D (hb)CB(cgcd)HDsample_3isotropicsample_conditions_1
2D (hb)CB(cgcdce)HEsample_3isotropicsample_conditions_1
4D (C13)HSQC-NOESY-(N15)HSQCsample_1isotropicsample_conditions_1
4D (C13)HSQC-NOESY-(C13)HSQCsample_3isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance III 600 MHz

Related Database Links:

BMRB 16677 18344 4081
PDB
DBJ BAJ20902
EMBL CAA23805 CAA23809 CAA23810 CAA25770 CAA46954
GB AAA52715 AAA59181 AAB59402 AAC64915 AAH74936
PRF 0611561A 0906342A
REF NP_000596 XP_003830724 XP_004047919 XP_528568
SP P01563