BMRB Entry 18125

Title:
Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms
Deposition date:
2011-12-08
Original release date:
2012-03-09
Authors:
Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu
Citation:

Citation: Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu. "Resonance assignments of the 56kDa chimeric avidin in the biotin-bound and free forms."  Biomol. NMR Assignments 7, 35-38 (2013).
PubMed: 22392339

Assembly members:

Assembly members:
avidin, polymer, 129 residues, Formula weight is not available
BTN, non-polymer, 244.311 Da.

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET101D

Data sets:
Data typeCount
13C chemical shifts418
15N chemical shifts120
1H chemical shifts851

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1avidin1
2BTN1

Entities:

Entity 1, avidin 129 residues - Formula weight is not available

1   GLNTHRVALALAARGLYSCYSSERLEUTHR
2   GLYLYSTRPTHRASNASPLEUGLYSERASN
3   METTHRILEGLYALAVALASNSERARGGLY
4   GLUPHETHRGLYTHRTYRILETHRALAVAL
5   ALAASPASNPROGLYASNILETHRLEUSER
6   PROLEULEUGLYILEGLNHISLYSARGALA
7   SERGLNPROTHRPHEGLYPHETHRVALASN
8   TRPLYSPHESERGLUSERTHRTHRVALPHE
9   THRGLYGLNCYSPHEILEASPARGASNGLY
10   LYSGLUVALLEULYSTHRMETTRPLEULEU
11   ARGSERSERVALASNASPILEGLYASPASP
12   TRPLYSALATHRARGVALGLYTYRASNILE
13   PHETHRARGLEUARGTHRGLNLYSGLU

Samples:

sample_1: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; d-biotin0.7 – 1.2 mM; H2O 93%; D2O 7%

sample_2: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 333 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 343 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
(HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
HCCmHmsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection, processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian Unity INOVA 800 MHz

Related Database Links:

BMRB 18123
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks