BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18098

Title: Solution NMR Structure of mitochondrial succinate dehydrogenase assembly factor 2 from Saccharomyces cerevisiae, Northeast Structural Genomics Consortium Target YT682A

Authors: Eletsky, Alexander; Lee, Hsiau-Wei; Lee, Dan; Kohan, Eitan; Hamilton, Keith; Acton, Thomas; Xiao, Rong; Everett, John; Prestegard, James; Montelione, Gaetano; Szyperski, Thomas

Citation: Eletsky, Alexander; Lee, Hsiau-Wei; Lee, Dan; Kohan, Eitan; Hamilton, Keith; Acton, Thomas; Xiao, Rong; Everett, John; Prestegard, James; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR Structure of mitochondrial succinate dehydrogenase assembly factor 2 from Saccharomyces cerevisiae"  To be published ., .-..

Assembly members:
YT682A, polymer, 109 residues, 13193.156 Da.

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
YT682A: MGHHHHHHSHMIKRTNEPLD KKRARLIYQSRKRGILETDL LLSGFAAKYLKKMNEEELEE YDSLLNELDWDIYYWATKNF KTSPLPDKWANSKLLKQLQE FSENKEKEI

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts111
1H chemical shifts784
residual dipolar couplings152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YT682A1

Entities:

Entity 1, YT682A 109 residues - 13193.156 Da.

1   METGLYHISHISHISHISHISHISSERHIS
2   METILELYSARGTHRASNGLUPROLEUASP
3   LYSLYSARGALAARGLEUILETYRGLNSER
4   ARGLYSARGGLYILELEUGLUTHRASPLEU
5   LEULEUSERGLYPHEALAALALYSTYRLEU
6   LYSLYSMETASNGLUGLUGLULEUGLUGLU
7   TYRASPSERLEULEUASNGLULEUASPTRP
8   ASPILETYRTYRTRPALATHRLYSASNPHE
9   LYSTHRSERPROLEUPROASPLYSTRPALA
10   ASNSERLYSLEULEULYSGLNLEUGLNGLU
11   PHESERGLUASNLYSGLULYSGLUILE

Samples:

NC: YT682A, [U-100% 13C; U-100% 15N], 1.0 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; sodium azide 0.02%; DSS 50 uM

NC5: YT682A, [5% 13C; U-100% 15N], 1.1 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; sodium azide 0.02%; DSS 50 uM

NC5-PEG: YT682A, [5% 13C; U-100% 15N], 0.7 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; sodium azide 0.02%; DSS 50 uM; C12E5 PEG 4%; hexanol 4%

NC5-GEL: YT682A, [5% 13C; U-100% 15N], 1.1 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; sodium azide 0.02%; DSS 50 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNCisotropicsample_conditions_1
2D 1H-13C CT-HSQC aliphaticNCisotropicsample_conditions_1
3D HNCONCisotropicsample_conditions_1
3D CBCA(CO)NHNCisotropicsample_conditions_1
3D HNCACBNCisotropicsample_conditions_1
3D HBHA(CO)NHNCisotropicsample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESYNCisotropicsample_conditions_1
3D HN(CA)CONCisotropicsample_conditions_1
2D 1H-13C CT-HSQC aromaticNCisotropicsample_conditions_1
3D (H)CCH-TOCSYNCisotropicsample_conditions_1
3D (H)CCH-COSY aliphaticNCisotropicsample_conditions_1
3D (H)CCH-COSY aromaticNCisotropicsample_conditions_1
1D 15N T1NCisotropicsample_conditions_1
1D 15N T2NCisotropicsample_conditions_1
2D 1H-13C CT-HSQC methylNC5isotropicsample_conditions_1
2D J-resolved 1H-15N HSQCNC5isotropicsample_conditions_1
2D J-resolved 1H-15N HSQCNC5-PEGanisotropicsample_conditions_1
2D J-resolved 1H-15N HSQCNC5-GELanisotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution, geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement, geometry optimization, structure solution

ASDP v1.0, Huang, Tejero, Powers and Montelione - data analysis, refinement

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - data analysis, chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY v1.3.13, Bartels et al. - data analysis

PROSA v6.4, Guntert - processing

VNMRJ v2.2D, Varian - collection

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

CARA v1.8.4, Keller and Wuthrich - data analysis,peak picking,chemical shift assignment

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ GAA26256
EMBL CAA99081 CAY86219
GB AHY77242 AJP41474 AJT70893 AJT71384 AJT71872
REF NP_014570
SP A6ZND9 B3LIY9 Q08230
TPG DAA10712