BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18096

Title: NMR structures of the transmembrane domains of the AChR b2 subunit   PubMed: 22361591

Authors: Bondarenko, Vasyl; Mowrey, David; Tillman, Tommy; Cui, Tanxing; Liu, Lu; Xu, Yan; Tang, Pei

Citation: Bondarenko, Vasyl; Mowrey, David; Tillman, Tommy; Cui, Tanxing; Liu, Lu Tian; Xu, Yan; Tang, Pei. "NMR structures of the transmembrane domains of the 42 nAChR."  Biochim. Biophys. Acta 1818, 1261-1268 (2012).

Assembly members:
entity, polymer, 137 residues, 14998.831 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: SNAEEEPLFYTINLIIPCVL ITSLAILVFYLPSDCGEKMT LCISVLLALTVFLLLISKIV PPTSSDSPSVGEYLMFTMVL VTFSIVTSVCVLNVHHRSPE THTGGGGGIDRLFLWIFVFV CVFGTIGMFLQPLFQEE

Data sets:
Data typeCount
13C chemical shifts552
15N chemical shifts127
1H chemical shifts820

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nAChR1

Entities:

Entity 1, nAChR 137 residues - 14998.831 Da.

1   SERASNALAGLUGLUGLUPROLEUPHETYR
2   THRILEASNLEUILEILEPROCYSVALLEU
3   ILETHRSERLEUALAILELEUVALPHETYR
4   LEUPROSERASPCYSGLYGLULYSMETTHR
5   LEUCYSILESERVALLEULEUALALEUTHR
6   VALPHELEULEULEUILESERLYSILEVAL
7   PROPROTHRSERSERASPSERPROSERVAL
8   GLYGLUTYRLEUMETPHETHRMETVALLEU
9   VALTHRPHESERILEVALTHRSERVALCYS
10   VALLEUASNVALHISHISARGSERPROGLU
11   THRHISTHRGLYGLYGLYGLYGLYILEASP
12   ARGLEUPHELEUTRPILEPHEVALPHEVAL
13   CYSVALPHEGLYTHRILEGLYMETPHELEU
14   GLNPROLEUPHEGLNGLUGLU

Samples:

sample_1: nAChR, [U-100% 13C; U-100% 15N], 0.25 mM; sodium acetate 5 mM; LDAO 1.5%; DSS 0.1 mM; D2O, [U-100% 2H], 5%; sodium chloride 10 mM; beta-mercaptoethanol 20 mM; H2O 95%

sample_conditions_1: pH: 4.65; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

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