BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18078

Title: NH chemical shift Assignments for AbpSH3 bound to WT Srv2 peptide (Srv17)   PubMed: 23251481

Authors: Stollar, Elliott; Forman-Kay, Julie

Citation: Stollar, Elliott; Lin, Hong; Davidson, Alan; Forman-Kay, Julie. "Differential Dynamic Engagement within 24 SH3 Domain: Peptide Complexes Revealed by Co-Linear Chemical Shift Perturbation Analysis."  PLoS ONE 7, e51282-e51282 (2012).

Assembly members:
AbpSH3, polymer, 59 residues, Formula weight is not available
Srv17, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
AbpSH3: APWATAEYDYDAAEDNELTF VENDKIINIEFVDDDWWLGE LEKDGSKGLFPSNYVSLGN
Srv17: KSGPPPRPKKPSTLKTK

Data sets:
Data typeCount
15N chemical shifts65
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AbpSH31
2Srv172

Entities:

Entity 1, AbpSH3 59 residues - Formula weight is not available

1   ALAPROTRPALATHRALAGLUTYRASPTYR
2   ASPALAALAGLUASPASNGLULEUTHRPHE
3   VALGLUASNASPLYSILEILEASNILEGLU
4   PHEVALASPASPASPTRPTRPLEUGLYGLU
5   LEUGLULYSASPGLYSERLYSGLYLEUPHE
6   PROSERASNTYRVALSERLEUGLYASN

Entity 2, Srv17 17 residues - Formula weight is not available

1   LYSSERGLYPROPROPROARGPROLYSLYS
2   PROSERTHRLEULYSTHRLYS

Samples:

AbpSH3-Srv17: AbpSH3, [U-100% 15N], 100 uM; sodium phosphate 50 mM; sodium chloride 100 mM; D2O 10%; H2O 90%; Srv17 600 uM

sample_conditions_1: ionic strength: 0.189 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCAbpSH3-Srv17isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 25354 26519
PDB
DBJ GAA21988 GAA25960
EMBL CAA36075 CAA42253 CAY78291 CAA86887 CAA96020 CAY82465
GB AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991 AAA35094 AAA63569 AHY76960 EDN62677 EDV12390
PRF 1603360A
REF NP_010012 NP_014261 XP_011105209
SP P15891 P17555
TPG DAA07557 DAA10410