BMRB Entry 18071

Title:
NH chemical shift Assignments for AbpSH3 bound to WT Prk1 peptide (ArkA_P(-1)A)
Deposition date:
2011-11-13
Original release date:
2013-01-03
Authors:
Stollar, Elliott; Forman-Kay, Julie
Citation:

Citation: Stollar, Elliott; Lin, Hong; Davidson, Alan; Forman-Kay, Julie. "Differential Dynamic Engagement within 24 SH3 Domain: Peptide Complexes Revealed by Co-Linear Chemical Shift Perturbation Analysis."  PLoS ONE 7, e51282-e51282 (2012).
PubMed: 23251481

Assembly members:

Assembly members:
AbpSH3, polymer, 59 residues, Formula weight is not available
Prk17, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts65
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AbpSH31
2Prk172

Entities:

Entity 1, AbpSH3 59 residues - Formula weight is not available

1   ALAPROTRPALATHRALAGLUTYRASPTYR
2   ASPALAALAGLUASPASNGLULEUTHRPHE
3   VALGLUASNASPLYSILEILEASNILEGLU
4   PHEVALASPASPASPTRPTRPLEUGLYGLU
5   LEUGLULYSASPGLYSERLYSGLYLEUPHE
6   PROSERASNTYRVALSERLEUGLYASN

Entity 2, Prk17 17 residues - Formula weight is not available

1   LYSSERARGPROPROARGPROPROPROLYS
2   PROLEUHISLEUARGTHRGLU

Samples:

AbpSH3-Prk17: AbpSH3, [U-100% 15N], 100 uM; sodium phosphate 50 mM; sodium chloride 100 mM; D2O 10%; H2O 90%; Prk17 257 uM

sample_conditions_1: ionic strength: 0.189 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCAbpSH3-Prk17isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18072 18073 18074 18075 18076 18077 18078 25354 26519
PDB
DBJ GAA21988 GAA24026
EMBL CAA36075 CAA42253 CAY78291 CAA86699 CAY80418
GB AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991 AAA86529 AHY75912 EDN61407 EDV09573 EDZ71531
PRF 1603360A
REF NP_010012 NP_012171
SP P15891 P40494
TPG DAA07557 DAA08458
AlphaFold P15891 P40494

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks