BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18055

Title: NH chemical shift assignments for free AbpSH3 bound to WT Ark1p peptide   PubMed: 23251481

Authors: Stollar, Elliott; Forman-Kay, Julie

Citation: Stollar, Elliott; Lin, Hong; Davidson, Alan; Forman-Kay, Julie. "Differential Dynamic Engagement within 24 SH3 Domain: Peptide Complexes Revealed by Co-Linear Chemical Shift Perturbation Analysis."  PLoS ONE 7, e51282-e51282 (2012).

Assembly members:
AbpSH3, polymer, 59 residues, Formula weight is not available
ArkA, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
AbpSH3: APWATAEYDYDAAEDNELTF VENDKIINIEFVDDDWWLGE LEKDGSKGLFPSNYVSLGN
ArkA: KKTKPTPPPKPSHLKPK

Data sets:
Data typeCount
15N chemical shifts65
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AbpSH31
2ArkA2

Entities:

Entity 1, AbpSH3 59 residues - Formula weight is not available

1   ALAPROTRPALATHRALAGLUTYRASPTYR
2   ASPALAALAGLUASPASNGLULEUTHRPHE
3   VALGLUASNASPLYSILEILEASNILEGLU
4   PHEVALASPASPASPTRPTRPLEUGLYGLU
5   LEUGLULYSASPGLYSERLYSGLYLEUPHE
6   PROSERASNTYRVALSERLEUGLYASN

Entity 2, ArkA 17 residues - Formula weight is not available

1   LYSLYSTHRLYSPROTHRPROPROPROLYS
2   PROSERHISLEULYSPROLYS

Samples:

AbpSH3-ArkA: AbpSH3, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; D2O 10%; H2O 90%; ArkA 1.05 mM

sample_conditions_1: ionic strength: 0.189 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCAbpSH3-ArkAisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 16843 18054 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 18078 25354 26519 16843 17223 18067
PDB
DBJ GAA21988 GAA26063
EMBL CAA36075 CAA42253 CAY78291 CAA95882
GB AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991 AHY77072 EDN62778 EDV12290 EEU04773 EGA56977
PRF 1603360A
REF NP_010012 NP_014378
SP P15891 P53974
TPG DAA07557 DAA10525