BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18028

Title: Solution NMR structure of CaM bound to the eNOS CaM binding domain peptide   PubMed: 22486744

Authors: Piazza, Michael; Futrega, Kathryn; Spratt, Donald; Guillemette, J.; Dieckmann, Thorsten

Citation: Piazza, Michael; Futrega, Kathryn; Spratt, Donald; Dieckmann, Thorsten; Guillemette, J.. "Structure and Dynamics of Calmodulin (CaM) Bound to Nitric Oxide Synthase Peptides: Effects of a Phosphomimetic CaM Mutation"  Biochemistry 51, 3651-3661 (2012).

Assembly members:
CaM, polymer, 148 residues, 16721.465 Da.
eNOS, polymer, 17 residues, 1838.215 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CaM: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
eNOS: KKTFKEVANAVKISASL

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts145
1H chemical shifts861

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM1
2eNOS2

Entities:

Entity 1, CaM 148 residues - 16721.465 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, eNOS 17 residues - 1838.215 Da.

1   LYSLYSTHRPHELYSGLUVALALAASNALA
2   VALLYSILESERALASERLEU

Samples:

sample_1: CaM, [U-99% 13C; U-99% 15N], 1.0 mM; entity_2 1.0 mM; potassium chloride 100 mM; calcium chloride 10 mM; sodium azide 0.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310 15185
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAA05652 BAD15356 BAD97356 BAF85617 BAG37648
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA53950 CAI10801
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA30494 AAA30667 AAA30669 AAA36364 AAA36365
PIR JC1305 MCON
PRF 0409298A 0608335A 2011304A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_000594 NP_001003158 NP_001076202 NP_001123373 NP_001153581
SP O02367 O16305 O96081 P02594 P05932 P29473 P29474 P70313 Q28969 Q62600
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA30266