BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18024

Title: 13C and 15N chemical shift assignments of cAMP bound Cyclic Nucleotide-Binding Domain from a bacterial Cyclic Nucleotide-Gated channel   PubMed: 22302441

Authors: Cukkemane, Abhishek

Citation: Cukkemane, Abhishek; Nand, Deepak; Gradmann, Sabine; Weingarth, Markus; Kaupp, U. Benjamin; Baldus, Marc. "Solid-state NMR [13C,15N] resonance assignments of the nucleotide-binding domain of a bacterial cyclic nucleotide-gated channel."  Biomol. NMR Assignments 6, 225-229 (2012).

Assembly members:
Cyclic nucleotide binding domain, subunit 1, polymer, 142 residues, Formula weight is not available
CMP, non-polymer, 329.206 Da.

Natural source:   Common Name: Mesorhizobium loti   Taxonomy ID: 381   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mesorhizobium loti

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cyclic nucleotide binding domain, subunit 1: GSQEVRRGDFVRNWQLVAAV PLFQKLGPAVLVEIVRALRA RTVPAGAVICRIGEPGDRMF FVVEGSVSVATPNPVELGPG AFFGEMALISGEPRSATVSA ATTVSLLSLHSADFQMLCSS SPEIAEIFRKTALERRGAAA SA

Data sets:
Data typeCount
13C chemical shifts434
15N chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cyclic nucleotide binding domain, subunit 11
2cyclic adenosine monophosphate2

Entities:

Entity 1, Cyclic nucleotide binding domain, subunit 1 142 residues - Formula weight is not available

1   GLYSERGLNGLUVALARGARGGLYASPPHE
2   VALARGASNTRPGLNLEUVALALAALAVAL
3   PROLEUPHEGLNLYSLEUGLYPROALAVAL
4   LEUVALGLUILEVALARGALALEUARGALA
5   ARGTHRVALPROALAGLYALAVALILECYS
6   ARGILEGLYGLUPROGLYASPARGMETPHE
7   PHEVALVALGLUGLYSERVALSERVALALA
8   THRPROASNPROVALGLULEUGLYPROGLY
9   ALAPHEPHEGLYGLUMETALALEUILESER
10   GLYGLUPROARGSERALATHRVALSERALA
11   ALATHRTHRVALSERLEULEUSERLEUHIS
12   SERALAASPPHEGLNMETLEUCYSSERSER
13   SERPROGLUILEALAGLUILEPHEARGLYS
14   THRALALEUGLUARGARGGLYALAALAALA
15   SERALA

Entity 2, cyclic adenosine monophosphate - C10 H12 N5 O6 P - 329.206 Da.

1   CMP

Samples:

sample_1: Cylic Nucleotide Binding Domain, [U-100% 13C; U-100% 15N], 10 mg; H2O 95%; D2O 5%; Tris 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 8; pressure: 1 atm; temperature: 263 K

Experiments:

NameSampleSample stateSample conditions
2D CCsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
2D NCACXsample_1solidsample_conditions_1
3D CCCsample_1solidsample_conditions_1

Software:

SPARKY, Bruker Biospin, Cornilescu, Delaglio and Bax, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 15249 16628
PDB
DBJ BAB50178
REF WP_010911524 WP_032931689
SP Q98GN8