BMRB Entry 18022

Title:
Structure of the Cx43 C-terminal domain bound to tubulin
Deposition date:
2011-10-26
Original release date:
2012-05-22
Authors:
Saidi Brikci-Nigassa, Amal; Clement, Marie-Jeanne; Ha-Duong, Tap; Benmansour, Kamal; Adjadj, Elisabeth; Ziani, Latifa; Pastre, David; Curmi, Patrick
Citation:

Citation: Saidi Brikci-Nigassa, Amal; Clement, Marie-Jeanne; Ha-Duong, Tap; Adjadj, Elisabeth; Ziani, Latifa; Pastre, David; Curmi, Patrick; Savarin, Philippe. "Phosphorylation controls the interaction of the connexin43 C-terminal domain with tubulin and microtubules."  Biochemistry 51, 4331-4342 (2012).
PubMed: 22558917

Assembly members:

Assembly members:
entity, polymer, 26 residues, 2720.094 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: KGVKDRVKGKSDPYHATSGA LSPAKD

Data sets:
Data typeCount
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cx431

Entities:

Entity 1, Cx43 26 residues - 2720.094 Da.

Swiss-Prot: P08050.2

1   LYSGLYVALLYSASPARGVALLYSGLYLYS
2   SERASPPROTYRHISALATHRSERGLYALA
3   LEUSERPROALALYSASP

Samples:

sample_1: Cx43 0.8 mM; D2O, [U-100% 15N], 0.04 mM; PIPES, [U-100% 15N], 50 mM; H2O 95%; D2O 5%

sample_2: Cx43 0.8 mM; D2O, [U-100% 15N], 0.04 mM; PIPES, [U-100% 15N], 50 mM; tubulin 0.04 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0 M; pH: 6.9; pressure: 1 atm; temperature: 282 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
1D STDsample_2isotropicsample_conditions_1
1D STDsample_1isotropicsample_conditions_1

Software:

NMRView v5.2, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAD97009 BAG35246 BAG57837 BAG58393 BAG59841
EMBL CAA37122 CAG46461
GB AAA52131 AAD37802 AAH26329 AAX29876 AAX36796
REF NP_000156 XP_002817351
SP P17302
AlphaFold P17302