BMRB Entry 17947

Title:
Partial backbone 1H assignments of the 2/2 hemoglobin from the cyanobacterium Synechococcus sp. PCC 7002 in the ferrous bis-histidine state
Deposition date:
2011-09-19
Original release date:
2012-05-02
Authors:
Lecomte, Juliette; Preimesberger, Matthew; Pond, Matthew; Majumdar, Ananya
Citation:

Citation: Preimesberger, Matthew; Pond, Matthew; Majumdar, Ananya; Lecomte, Juliette. "Electron self-exchange and self-amplified posttranslational modification in the hemoglobins from Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002"  J. Biol. Inorg. Chem. 17, 599-609 (2012).
PubMed: 22349976

Assembly members:

Assembly members:
GlbN, polymer, 123 residues, Formula weight is not available
HEB, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: cyanobacteria synechococcus   Taxonomy ID: 269084   Superkingdom: Eukaryota   Kingdom: Cyanobacteria   Genus/species: Synechococcus Synechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3c

Data sets:
Data typeCount
15N chemical shifts115
1H chemical shifts249
heteronuclear NOE values91
T1 relaxation values92
T2 relaxation values93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlbN1
2HEB2

Entities:

Entity 1, GlbN 123 residues - Formula weight is not available

1   ALASERLEUTYRGLULYSLEUGLYGLYALA
2   ALAALAVALASPLEUALAVALGLULYSPHE
3   TYRGLYLYSVALLEUALAASPGLUARGVAL
4   ASNARGPHEPHEVALASNTHRASPMETALA
5   LYSGLNLYSGLNHISGLNLYSASPPHEMET
6   THRTYRALAPHEGLYGLYTHRASPARGPHE
7   PROGLYARGSERMETARGALAALAHISGLN
8   ASPLEUVALGLUASNALAGLYLEUTHRASP
9   VALHISPHEASPALAILEALAGLUASNLEU
10   VALLEUTHRLEUGLNGLULEUASNVALSER
11   GLNASPLEUILEASPGLUVALVALTHRILE
12   VALGLYSERVALGLNHISARGASNASPVAL
13   LEUASNARG

Entity 2, HEB - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEB

Samples:

sample_1: GlbN, [U-15N], 1.6 mM; HEB 1.6 mM; potassium phosphate 100 mM; glucose 0.1%; glucose oxidase 0.2-0.5 mg/mL; catalase 0.05-0.2 mg/mL; dithionite 8 mM

sample_2: GlbN, [U-100% 13C; U-100% 15N], .76 mM; HEB .76 mM; potassium phosphate 250 mM; glucose 0.1%; glucose oxidase 0.2-0.5 mg/mL; catalase 0.05-0.2 mg/mL; dithionite 4 mM

sample_3: GlbN, [U-100% 15N], 1.6 mM; HEB 1.6 mM; potassium phosphate 100 mM; glucose 8 mM; glucose oxidase 0.8 uM; catalase 0.05 uM

sample_conditions_1: ionic strength: 0.3 M; pH: 7.15; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.75 M; pH: 7.15; pressure: 1 atm; temperature: 298 K

sample_conditions_3: ionic strength: 0.3 M; pH: 7.20; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N NOEsample_3isotropicsample_conditions_3
2D 15N R1sample_3isotropicsample_conditions_3
2D 15N R2sample_3isotropicsample_conditions_3
2D 1H-15N HSQCsample_3isotropicsample_conditions_3

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 16306 16307 18422 18423 18424
PDB
GB AAL79195 ACA99611
REF WP_012307234 WP_030006991